肝移植后胆道铸型4例全蛋白质的表达(英文)  

Holoprotein expression in four biliary cast syndrome patients after liver transplantation

作　　者：朱晓丹 沈中阳 臧运金 刘峰[2] 王鸿丽[2] 

机构地区：[1]武装警察部队总医院肝脏移植研究所,北京市100039 [2]解放军军事医学科学院生物医学分析中心,北京市100850

出　　处：《中国组织工程研究与临床康复》2010年第18期3401-3404,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:肝移植后胆道铸型综合征的胆道铸型形成机制尚未彻底被阐明,因此胆道铸型中是否存在一些和其形成有关或对铸型形成有预警作用的蛋白质还不清楚。目的:了解肝移植后胆道铸型综合征患者胆道铸型中全蛋白质表达情况,总结其规律。方法:武警总医院肝移植研究所实施肝移植患者4例,于移植后3个月经T管窦道行纤维胆道镜检查治疗时取出胆道铸型,将4种不同颜色质地的胆道铸型深低温保存,经过蛋白质提取、酶切,最后经高解析离子淌度质谱仪鉴定并经MASCOT数据库查询,得出每份标本的全蛋白质名称。比较4份标本的差异,找出共同的蛋白质。结果与结论:通过检测及查询4份标本分别包含82、44、56及65种蛋白质,经比对分析,重叠4份标本的共同蛋白质13种,除此外,重叠3份标本的蛋白质7种、重叠2份标本的蛋白质5种。重叠4份标本共同蛋白质中已命名蛋白质8种,未命名蛋白质5种,命名蛋白质包括:α-纤维蛋白原前体、β-纤维蛋白原前体、纤维蛋白原γ链、载脂蛋白A链、人组织蛋白酶G、钙结合蛋白A9和乳铁蛋白等。证实胆道铸型中确实存在蛋白质,4例胆道铸型的共同蛋白质揭示了胆道铸型的形成与胆道上皮受损后的胆道渗出性炎症(纤维蛋白渗出)有关;一些蛋白质有可能作为预警胆道铸型综合征的出现、判定胆道上皮受损后胆道炎症程度及胆道铸型综合征预后的标记物。BACKGROUND：The formation mechanism of biliary cast syndrome following liver transplantation has not been thoroughly illuminated,and it is unclear that whether some proteins correlated to the formation mechanism of biliary cast or prewarning to the formation of biliary cast.OBJECTIVE：To investigate the holoprotein expression in biliary cast syndrome patients following liver transplantation.METHODS：Four patients underwent liver transplantation at Liver Transplantation Institute,General Hospital of Chinese People＇s Armed Police Force.Three months later,10 g biliary cast was harvested.Four kinds of biliary cast specimens at different colors and textures were preserved at deep hypothermia,followed by protein abstraction and restriction enzyme digestion,the total protein abstraction solution of biliary cast were analyzed by high definition mass spectrometry and query on MASCOT database.All protein name of biliary cast were list,the conjunct protein was found by comparing 4 specimens.RESULTS AND CONCLUSION：There were totally 208 proteins in 4 biliary cast specimens,82,44,56 and 65,respectively.By comparison,5 proteins were found to overlay in 2 biliary cast specimens,7 proteins in 3 specimens and 13 proteins in 4 specimens.Among the latter 13 proteins,5 unnamed-proteins,as well as 8 named-proteins（termed alpha-fibrinogen precursor,beta-fibrinogen precursor,fibrinogen gamma chain,proapolipoprotein,Chain A of Human Cathepsin G,S100 calcium-binding protein A9,lactoferrin）were included.The proteins exists in biliary cast,the common proteins of 4 biliary cast specimens imply a correlation between the formation of biliary cast and the exudative inflammation following the damage of biliary tract epithelium;Some proteins might be considered as a marker of prewarning the presence of biliary cast syndrome,judging the inflammation severity following the damage of biliary tract epithelium and the prognosis of biliary cast syndrome.

关 键 词：胆道铸型 肝移植 蛋白分析 全蛋白质 

分 类 号：R737.9[医药卫生—肿瘤]