雌、孕激素对子宫内膜癌细胞株HEC-1B ERRα表达调控的研究  

Regulation of ERRα by estrogen and progestogen in endometrial carcinoma cell line

作　　者：丁岩[1] 徐怀胜[1] 王丽莉[1] 辛卫娟[1] 吴乾渝[1] 

机构地区：[1]同济大学附属东方医院妇产科,上海200120

出　　处：《现代妇产科进展》2010年第3期202-205,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨ERRα与雌、孕激素的相互作用及其在ERα阴性子宫内膜癌发病中的作用。方法:用MTT、实时定量PCR方法,检测不同浓度雌二醇(E2)、孕酮(P)作用于HEC-1B细胞后细胞增殖和ERRα mRNA的变化。结果:E2促进细胞增殖,E2(10-6～10-8mol/L)组OD值与对照组相比在48h时有统计学差异(P<0.05);E2明显上调ERRα表达,且10-7mol/L时上调最明显,Pearson相关分析ERRα mRNA表达含量与相应细胞生长变化显示:24、48h呈正相关(P<0.05),表明雌激素通过上调ERRα表达而诱导细胞增殖。P抑制细胞增殖,并具有浓度和时间依赖性,P(10-4～10-6mol/L)组OD值与对照组相比差异有统计学意义(P<0.05);P明显下调ERRα表达,且10-4mol/L时下调最明显,Pearson相关分析ERRαmRNA表达含量与相应浓度细胞生长变化在24h呈正相关(P<0.05),表明孕激素通过下调ERRα表达而抑制细胞增殖。结论:ERRα在ERα阴性子宫内膜癌的发生中发挥一定作用。Objective：To explore the relationship between the expressions of ERRα mRNA and estrogen,progesterone,and to elucidate the function of ERRα in the endometrial carcinoma.Methods：HEC-1B cells were dealt with different concentrations of β-estradiol and progesterone respectively.The proliferation of the cells was measured by MTT assay.The expression levels of ERRα mRNA were examined by real-time quantitive PCR.Results：E2 could promote the proliferation of HEC-1B cells,and the OD values in the groups of high concentration of E2（10-6～10-8mol/L） were increased compared with the control group（P0.05）.The expression levels of ERRα mRNA were significantly up-regulated after stimulated by different concentrations of E2.The maximal effect was observed at the concentration of 10-7mol/L.Progesterone could inhibit the proliferation of HEC-1B cells,which was enhanced with the increasing of dose and treatment time.And the OD values in the group of high concentration of progesterone（10-4～10-6mol/L） were decreased compared with control group（P0.05）.The expression levels of ERRα mRNA were significantly down-regulated after stimulated by different concentrations of progesterone.The maximal effect was observed at the concentration of 10-4mol/L.Positive correlations were found between the proliferation of HEC-1B cells and the expression levels of ERRα mRNA after stimulated by different concentration of E2 and progesterone,which means estrogen could promote the cell proliferation by up regulating the expression levels of ERRα mRNA,while progesterone could inhibit the cell proliferation by down regulating the expression levels of ERRα mRNA in the ERα-negative endometrial carcinoma cell line HEC-1B.Conclusion：ERRα may play a role in the development of endometrial carcinoma.

关 键 词：雌激素受体相关受体 子宫内膜肿瘤 雌激素类 孕激素类 

分 类 号：R737.33[医药卫生—肿瘤]