华支睾吸虫病与原发性肝(胆管)癌关系的动物实验研究和临床病例观察  

Animal Experimental Study and Clinical Observation on Relation between Clonorchiasis and Primary Liver Carcinoma

作　　者：王磊[1] 唐永煌[1] 肖锡昌[1] 曹雅鲁[1] 

机构地区：[1]暨南大学医院第一附属医院感染科

出　　处：《暨南大学学报（自然科学与医学版）》1998年第6期59-60,共2页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：报道用华支睾吸虫与二甲基亚硝胺诱发动物肝癌的实验研究和临床１４例华支睾吸虫病并原发性肝癌病例的观察。在动物实验中，Ａ组每只金地鼠通过胃管人工感染华支睾吸虫囊蚴３０ｄ后当鼠粪中查见华支睾吸虫卵时开始自由饮服质量分数为０００２５％二甲基亚硝胺溶液１７周。结果１１只鼠中４只诱发肝癌、１只诱发肝胆管癌和５只发生肝硬变。Ｂ组每只鼠仅自由饮服质量分数为０００２５％二甲基亚硝胺溶液１７周，１５只鼠中，３只诱发肝癌和５只发生肝硬变。Ｃ组每只鼠仅通过胃管人工感染华支睾吸虫囊蚴，不饮服二甲基亚硝胺，１２只鼠中，仅６只发生肝硬变。Ｄ组空白对照的１５只鼠均未发现病变。结果显示金地鼠饮服二甲基亚硝胺可引起肝细胞和胆管细胞恶变，而华支睾吸虫在肝内的寄生可促进肝癌的发生。除本实验外，在世界上尚未看到由华支睾吸虫诱发肝胆管癌成功的实验。在临床１４例观察中，全部病例的大便中均找到华支睾吸虫虫卵，经临床和实验室检查，１２例诊断为原发性肝癌，２例诊断为胆管癌。本组１４例中５例经外科手术切除肿瘤及送病理检查，证实４例华支睾虫感染并肝胆管腺癌和（或）肝胆管上皮癌，１例华支睾吸虫感染并原发性肝细胞性肝癌。另５例肝穿刺活检，病理报告２例肝细胞性肝癌?This paper reports results of the experimental animal liver carcinoma induced by Clonorchis sinensis and dimethyl-nitrosamine(DMN)and results of the clinical observation on 14 cases of primary liver carcinoma induced by clonorchisasis.Among the experiment of animal,Goladen hamsters in the group A were infected artificially with metacercaria of Clonorchis sinensis via gastric tube.Thirty days later,when ova of the flukes appeared in the stool,golden hamsters were allowed to drink 0 0025% DMN solution freely for 17 weeks.The result showed that,of 11 hamsters,four developed liver carcinoma,one had intrahepatic bile duct carcinoma,and five had cirrhosis.In the group B,each hamster only drank 0 0025% DMN solution freely for 17 weeks.As the result,of 15 hamsters,3 had liver carcinoma and 5 had cirrhosis.Hamsters in group C were only treated with metacercaria of Clonorchis sinensis via the gastric tube. The result showed that, half of 12 hamsters had cirrhosis. In the control group,no pathological changes could be found in the liver of all 15 hamsters.Our results indicated that drinking DMN solution may cause malignant changes of hepatocyte and bile duct epithelial cells in hamster and parasitic Clonorchis sinensis may promote the formation of liver carcinoma.This animal experiment that Clonorchis sinensis causes hepatic cholangiocarcinoma is the first successful experiment in the world. The ova of flukes were found in the stool of 14 cases clinically observed from the examination of clinic and laboratory,twelve of these were diagnosed primary liver carcinoma,two of these were diagnoed cholangiocarcinoma.Tumour mass resected from five of these cases had examined by pathology.Four of the five cases had clonorchiasis and intrahepatic bile duct carcinoma and/or cholangiocarcinoma of bile duct epithelium.The other one case had clonorchiasis and primary liver carcinoma.Another five of the 14 cases had been done by needle biopsy, two of them had primary liver carcinoma,one of them had hyperplasia of bile duc

关 键 词：华支睾吸虫病 原发性肝癌 动物实验 胆管癌 

分 类 号：R735.7[医药卫生—肿瘤] R532.23[医药卫生—临床医学]