肌醇六磷酸诱导HT-29细胞凋亡及相关基因表达  

Effect of inositol hexaphosphate on apoptosis of HT-29 human colon carcinoma cells and expression of apoptosis related gene

作　　者：宋莉[1] 冷传理[2] 宋扬[1] 

机构地区：[1]青岛大学医学院营养研究所,山东青岛266021 [2]青岛大学医学院附属青岛市海慈医院

出　　处：《中国公共卫生》2010年第4期419-420,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30671767);山东省自然科学基金(2007BS03042)

摘　　要：目的研究肌醇六磷酸(IP6)对人结肠癌细胞系(HT-29)的凋亡诱导作用。方法将不同浓度IP6以不同时间作用于HT-29细胞,运用透射电镜观察IP6对HT-29细胞凋亡作用;流式细胞仪检测细胞凋亡率变化;钙离子荧光探针(Fura-2/AM)法测定细胞内Ca2+浓度变化;RT-PCR法检测细胞内caspase-3 mRNA表达。结果IP6诱导HT-29细胞发生凋亡的形态学变化为染色体固缩,可见凋亡小体;3.3mmol/L IP6作用6个月HT-29细胞凋亡率为4.4%,细胞内Ca2+浓度为38.66 mmol/L,与对照组比较明显升高(P<0.05);各IP6组Caspase-3mRNA表达均升高(P<0.05);IP6诱导HT-29细胞凋亡作用及Ca2+、Caspase-3mRNA的表达变化具有时间依赖关系。结论IP6对HT-29细胞有诱导凋亡作用,其机制可能是通过调节细胞内Ca2+浓度及Caspase-3的表达来实现。Objective To investigate effect and mechanism of inositol hexaphosphate（IP6） on HT-29 human colon carcinoma cell line.Methods HT-29 cells were exposed to various concentrations of IP6 for different time.The effect of apoptosis was detected by transmission electron microscopy（TEM）.Flow cytometric analysis was performed to detect cell apoptosis.Fura-2/AM was used to determine the concentration of intracellular-free calcium.Caspase-3 mRNA expression in cells was determined by reverse transcription polymerase chain reaction（RT-PCR）.Results Morphological examination showed cells shrinkage and the presence of ＂apoptopic bodies＂.IP6 increased the rate of cell apoptosis with a time-dependent trend（P0.05）,the concentration of intracellular-free calcium（P0.05）,and the expression of Caspase-3 mRNA（P0.05）.Conclusion IP6 could induce HT-29 cell apoptosis.Its mechanism may relates to the regulation of intracellular-free calcium and the expression of apoptosis related gene.

关 键 词：肌醇六磷酸 结肠癌 HT-29细胞系 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]