乙型肝炎病毒增强子Ⅰ区核苷酸序列变异分析  

Mutation in the Enhancer Ⅰ of HBV DNA in the Patients with Chronic Hepatitis B Virus

作　　者：姚全良[1] 刘金辉[2] 刘宁[1] 

机构地区：[1]南昌大学第二附属医院检验科,南昌330006 [2]南昌大学医学院微生物教研室,南昌330006

出　　处：《江西医学院学报》2009年第12期36-38,共3页Acta Academiae Medicinae Jiangxi

摘　　要：目的研究慢性乙型肝炎患者血液中乙型肝炎病毒增强子Ⅰ区基因突变状况。方法收集慢性乙型肝炎患者7例血清标本,抽提血清中HBVDNA,扩增HBVEnhⅠ基因,PCR产物测序后利用Gengbank中Genotyping对HBV进行分型,并分析核苷酸序列中的突变位点。结果1例标本因PCR产物太弱,无法满足测序要求,其他6例测序标本经Genotyping比对,均属于B型,与参考序列AF100309同源性最高;发现HBVEnhⅠ共有17个突变点,分别为A970T、G973A、T991C、A994G、C1013T、A1042T、G1099A、C1127T、G1180C、A1182C、T1201A、G1204A、C1206A、C1211G、A1249C、A1309G、A1330C。结论慢性乙型肝炎患者中HBVEnhⅠ基因选择性突变,可能下调HBV基因表达,是导致乙型肝炎慢性化的原因之一。Objective To analyze the mutation sites of EnhⅠ of hepatitis B virus DNA in patients with chronic infection of hepatitis B.Methods The serum samples of 7 patients with chronic hepatitis were collected.DNA was extracted from sera,and the EnhⅠ region was amplified by PCR.The PCR products were sequenced and used to analyzed the mutation sites by Sequence Alignment with Genotyping in Genebank.Results One cases of samples failed to sequence because of week PCR product.The results with Sequence Alignment with Genotyping showed that other six sequenced HBV DNA belonged to type B and were homologous to the reference sequence AF100309.Seventeen mutation sites were found in HBV EnhⅠ region and they were A970T、G973A、T991C、A994G、C1013T、A1042T、G1099A、C1127T、G1180C、A1182C、T1201A、G1204A、C1206A、C1211G、A1249C、A1309G、A1330C.Conclusion The mutation in the EnhⅠ could downregulate the expression of HBV genes,which is a possible mechanism leading to chronic hepatitis B.

关 键 词：乙型肝炎病毒 增强子 基因突变 

分 类 号：R342.4[医药卫生—基础医学] R512.62