VEGF促进大鼠正畸牙牙周组织改建的研究  

Effects of periodontal tissue remodeling of orthodontic tooth in rats with VEGF

作　　者：管晓燕[1] 徐宇红[2] 彭彩刚[1] 刘建国[1] 梁颖[1] 张疆弢[1] 苏牧[1] 黄瑾[1] 

机构地区：[1]遵义医学院口腔学院正畸学教研室,贵州遵义563000 [2]遵义医学院附属第五(珠海)医院口腔科,广东珠海519100

出　　处：《遵义医学院学报》2009年第6期562-566,共5页Journal of Zunyi Medical University

基　　金：贵州省自然科学基金项目(编号:黔科合J字[2009]2177号)

摘　　要：目的探讨局部应用外源性重组人血管内皮生长因子(rhVEGF)对大鼠正畸牙牙周组织改建的促进作用。方法建立SD大鼠正畸牙移动模型,将30只雄性SD模型大鼠按rhVEGF浓度不同随机分为:0μg/ml(对照组)、0.1μg/ml组、0.5μg/ml组、1μg/ml组、1.5μg/ml组和2μg/ml组;每组5只,隔日在正畸牙颊侧牙龈粘膜下注射不同浓度VEGF,注射体积为0.1ml,第10天处死大鼠,取正畸牙及其牙周组织作HE染色,观察牙周组织学改变,进行破骨细胞计数。结果随VEGF浓度增加,牙周组织改建逐渐活跃,之后逐渐下降。压力侧以破骨细胞活动为主,0.5μg/ml组破骨细胞计数最多,骨吸收最活跃(P<0.01)。结论一定浓度范围内的外源性VEGF可以加速正畸牙牙周骨组织的吸收。Objective To study the effects of rhVEGF on periodontal tissue remodeling of orthodontic tooth in rats. Methods Establishing orthodontic tooth movement model in male Sprague-Dawley rats： the first maxillary molar of the rats were moved medially by a elastic string delivering a force of 50g. 30 SD rats were randomly divided into 6 groups of five rats, in control group, the rats were injected with PBS （Phosphate-buffered saline） （0. 1ml） in the buceal submucosal area of d,e molar pertriduum, and VEGFgroups were injected with VEGF （0. 1ml） with concentrations of 0.1μg/ml,0.5μg/ml, 1μg/ml, 1.5μg/ml,2.0μg/ml.10 days late, all rats were killed and the first molars and periodontal tissues were chosen forhistoiogical evaluation. Results The activity of osteoclast increased in the level 0.5μg/ml nd in compression side in VEGF groups.Conelusions Exogenous VEGF can accelerate orthodontic periodontal bone resorption in rats.

关 键 词：血管内皮生长因子 正畸牙 牙周组织 大鼠 

分 类 号：R783[医药卫生—口腔医学]