载脂蛋白B基因多态性在心脑血管疾病低发的云南德宏傣族中的分布情况  

Distribution of Apolipoprotein B gene polymorphism in low cardiocerebral vascular disease zone of Yunnan Dehong Dai population

作　　者：唐慧[1] 华映坤[1] 董虹[1] 卫明荣 严新民[1] 

机构地区：[1]云南省第一人民医院（昆明医学院附属昆华医院）临床基础医学研究所云南省重点实验室,650032 [2]德宏景颇族傣族自治州医疗集团,678400

出　　处：《中国保健营养（下半月）》2009年第12期14-16,共3页China Health Care & Nutrition

基　　金：基金项目：云南省科技厅昆明医学院应用基础研究联合专项基金,No.2008CD044

摘　　要：目的探讨载脂蛋白（apolipoprotein，Apo）B基因的Xbal和EcoRI酶切多态性在心脑血管疾病低发的云南德宏傣族人群中的分布情况。方法在云南德宏和昆明两地随机抽样164名傣族和74名汉族人群，提取基因组DNA后，应用聚合酶链反应一限制性片段长度多态性（PCR—restrictionfragmentIengthpolymorphism，PCE--BFLP）技术检测ApoB基因的Xbal和Ecogl酶切多态性。结果ApoB／Xbal的等位基因×＋，×-；ApoB／EcoPq的等位基因E＋，E-在傣汉两民族中的分布频率依次为：傣族：0037，0963、0930、0070；汉族：0．054、0．946、0.951、0049。ApoB基因的4个等位基因频率在傣汉两民族间无显著性差异（P〉0.05）。结论ApoB的Xbal与EcoRI酶切多态性不是德宏傣族人群具有较低心脑血管疾病患病率的易感因子。傣族人群具有较低的心脑血管疾病患病率应归因于遗传因素和环境因素协同作用的结果。Objective To investigate XbaI and EcoRI digestion polymorphisms of apolipoprotein （Apo） B gene in Yunnan Dehong Dai population which has been reported have low cardiocerebral vascular disease rate. Methods A total of 164 endogamous subjects of Chinese Dehong Dai and 74 subjects of Chinese Kunming Han were surveyed by a stratified randomized cluster sampling. After extracting genomic DNA, the PCR-RFLP technique was used to investigate XbaI and EcoRI digestion polymorphisms of ApoB gene. Results The distribution of allelic frequencies of ApoB/XbaI （X＋, X-） and ApoB/EcoRI （E＋, E-） in Dehong Dai and Kunming Han were as following： Dai： 0.037, 0.963, 0.930, 0.070. Hart： 0. 054, 0.946, 0.951, 0.049, respectively. There have no significantly differences between Chinese Dehong Dai and Kunming Han on these 4 allele frequencies （P 〉 0.05）. Conclusions XbaI and EcoRI digestion polymorphisms of ApoB were not the susceptible factors of cardiocerebral vascular disease in Dehong Dai population. It was suggested that synergetic effect of genetic and environmental factors may contribute to the low cardiocerebral vascular disease rate in Yunnan Dehong Dai population.

关 键 词：载脂蛋白B 限制性片段长度多态性 德宏傣族 基因型频率 等位基因频率 

分 类 号：Q343.12[生物学—遗传学]