rAd5／NR2B镇痛疫苗对神经病理性痛大鼠认知功能的影响  

Effect of recombinant adenovirns serotype 5-mediated NR2B gene transfer on cognitive function in a rat of neuropathic pain

作　　者：杨少兵[1] 王公明[2] 杨辉[1] 高峰[1] 田学愎[1] 刘希江[1] 罗婷[1] 田玉科[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉学教研室,武汉市430030 [2]山东省立医院麻醉科

出　　处：《中华麻醉学杂志》2010年第2期162-164,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30471660）

摘　　要：目的评价N-甲基-D天冬氨酸受体2B亚基（NR2B）基因重组腺病毒（rAd5／NR2B）对神经病理性痛大鼠认知功能的影响。方法雌性SD大鼠40只，体重180～200g，随机分为4组（n=10）：对照组（C组）、rAd5／NR2B镇痛免疫组（rAd5／NR2B组）、神经病理性痛组（SP组）和rAd5／NR2B镇痛疫苗＋神经病理性痛组（rAd5／NR2B＋SP组）。C组和rAd5／NR2B组分别胃内灌注生理盐水0．1ml和rAd5／NR2B镇痛疫苗1×10^8 PFU，2周后重复灌注。SP组制备神经病理性痛模型，1周后腹腔注射生理盐水0．1ml。rAd5／NR2B＋SP组胃内灌注rAd5／NR2B镇痛疫苗1×10^8 PFU，2周后重复灌注，再1周后制备神经病理性痛模型。测定大鼠机械缩爪阈值（MWT）。行Morris水迷宫实验测定大鼠认知功能，记录潜伏期和游泳速度。采用免疫组织化学法检测大鼠海马NR2B的表达水平。结果（1）与C组比较，rAd5／NR2B组MWT差异无统计学意义（P〉0．05），SP组和rAd5／NR2B＋sP组MWT降低（P〈0．05）；与SP组比较，rAd5／NR2B＋SP组MWT升高（P〈0．05）。（2）各组游泳速度比较差异无统计学意义（P〉0．05）；与C组比较，rAd5／NR2B组潜伏期差异无统计学意义（P〉0．05），SP组和rAd5／NR2B＋SP组潜伏期延长（P〈0．05）；与SP组比较，rAd5／NR2B＋SP组潜伏期差异无统计学意义（P〉0．05）。（3）与C组比较，rAd5／NR2B组和rAd5／NR2B＋SP组海马NR2B表达差异无统计学意义（P〉0．05），SP组海马NR2B表达上调（P〈0．05）；与SP组比较，rAd5／NR2B＋SP组海马NR2B表达下调（P〈0．05）。结论rAd5／NR2B疼痛疫苗对神经病理性痛大鼠认知功能无明显影响。Objective To investigate the effect of recombinant adenovirus serotype 5-mediated NR2B gene transfer （rAd5/NR2B） on spatial learning and memory in a rat model of neuropathie pain. Methods Forty female SD rats weighing 180-200 g were randomly divided into 4 groups （n = 10 each） ： Ⅰ control group （group C） ; Ⅱ rAd5/NR2B group; Ⅲsciatic nerve injury group （group SNI） and Ⅳ rAd5/NR2B ＋ SNI group. In group C and rAd5/NR2B normal saline （NS） and rAd5/NR2B were administered by intragastrie gavage respectively. In group SNI NS was injected intraperitoneally after SNI. In rAd5/NR2B ＋ SNI group rAd5/NR2B was given by intragastric gavage before SNI. Mechanical paw-withdrawal threshold （MWT） was measured. All rats underwent Morris water maze test 3 times a day for 6 consecutive days. The animals were then killed. The expression of NR2B in hippoeampus was detected by immuno-histochemistry. Results （ 1 ） There was no significant difference in MWT between control group and rAd5/NR2B group. MWT was decreased in SNI and SNI ＋ rAd5/NR2B group as compared with control group. MWT was increased in SNI ＋ rAd5/NR2B group as compared with SNI group. （2）There was no significant difference in the escape time between control group and rAd5/NR2B group. The escape time was prolonged in SNI and SNI ＋ rAd5/NR2B group as compared with control group. There was no significant difference in escape time between SNI group and SNI ＋ rAd5/NR2B group. There was no significant difference in swimming velocity among the 4 groups. （3） There was no significant difference in the expression of NR2B in hippocampus between control group and rAd5/NR2B group. The NR2B expression was up-regulated in group SNI as compared with control group. The NR2B expression was down-regulated in SNI ＋ rAdS/NR2B group as compared with SNI group. Conclusion rAd5/NR2B does not significantly affect cognitive function in a rat model of neuropathic pain.

关 键 词：受体 N-甲基-D天冬氨酸 腺病毒科 神经痛 认知障碍 

分 类 号：R741[医药卫生—神经病学与精神病学]