不同配体硅胶弱阳离子交换剂的再生与应用  

Recovery and Regeneration of Weak Cation Exchange Agent Based on Silica with Different Ligands

作　　者：王燕[1] 张番[1] 李蓉[1] 陈国亮[1] 王小刚[1] 

机构地区：[1]西北大学化工学院,陕西西安710069

出　　处：《食品科学》2010年第7期67-70,共4页Food Science

基　　金：陕西省自然科学基金项目(2007B22);陕西省教育厅自然科学专项项目(09JK758);陕西省重点学科建设项目;西北大学校基金项目(U5NW4);西北大学研究生科研实验项目(09YSY24)

摘　　要：将不同配体的硅胶填料回收酸洗后,以亚氨基二乙酸(IDA)为新配体,分别用直接法和间接法合成IDA-回收硅胶,研究合成填料的色谱性能,并与制备的IDA-新硅胶色谱柱进行比较。结果表明,尽管IDA-回收硅胶柱的各项性能均略低于新硅胶色谱柱,但也能有效分离蛋白。用直接法合成得到的IDA-回收硅胶柱的性能要比用间接法合成得到的IDA-回收硅胶柱的性能好,并且成功的提取和纯化了蛋清中的溶菌酶(Lys)和猪心中的细胞色素-C(Cyt-C)。研究证明了硅胶回收再利用的可行性。Used silica with different ligands was recovered and regenerated by means of acid treatment.Iminodiacetic acid（IDA）recycled silica column was synthesized using IDA as new ligand and pickled silica with different ligands as matrix by direct or indirect methods.The chromatographic performance of synthesized packing materials was investigated.Results indicated that IDA-recycled silica column exhibited an excellent separation efficiency to proteins although its chromatographic performance was lower.IDA-recycled silica column synthesized by direct method revealed much better chromatographic performance than indirect synthesis method.The IDA-recycled silica column was successfully used to extract and purify lysozyme and cytochrome C from egg and pig heart,respectively.These investigations demonstrate the feasibility for recovery and regeneration of silica.

关 键 词：酸处理 直接法 间接法 IDA-回收硅胶柱 蛋白分离 

分 类 号：O647.316[理学—物理化学] TQ425.69[理学—化学]