用特征功能模块法挖掘一个虚寒证家系的基因表达谱  

Mining gene expression profi le in def icient cold syndrome with characteristics functional modules

作　　者：杨丽萍[1] 黄睿[1] 张洛欣[2] 王米渠[3] 吴伟 

机构地区：[1]河南中医学院生物化学与分子生物学学科,郑州450008 [2]新加坡国立大学数学系,新加坡117543 [3]成都中医药大学中医遗传学研究室,成都610075 [4]新加坡细胞分子生物学研究所,新加坡138673

出　　处：《中华中医药杂志》2010年第5期683-685,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金海外青年学者合作项目(No.30528029);河南省医学创新人才工程项目(No.2005075);河南中医学院博士基金项目(No.2007005)~~

摘　　要：目的:利用基因表达谱与基因功能知识识别虚寒证相关的差异基因与功能模块,从功能模块水平研究虚寒证发生的分子机制。方法:用Array Tools筛选差异表达基因,并检验分类效果,再利用Gene Ontology(基因本体,GO)工具,将特征基因进行功能注释,选择显著聚集差异表达基因的功能模块;识别其中能够有效分类疾病样本的特征功能模块,并分析它们与虚寒证的关联。结果:用25个差异基因分类样本准确率在93%,用11个功能模块分类样本准确率在87%-100%之间。结论:这些特征功能模块与虚寒证的发生有一定的关联性。基于特征功能模块法研究基因表达谱更有利于对疾病的阐释。Objective：To identify the deficient cold syndrome relevant genes and functional modules,based on gene expression profiles and gene functional knowledge to study the molecular mechanism of deficient cold syndrome.Methods：Screening the differential expression genes with Array Tools,and testing the classificational results to the samples.The Gene Ontology Tool was used for annotating characteristics functional gene,selecting functional modules enriched with differentially expressed genes;and then identifing the feature modules and analyzing their association with the deficient cold syndrome.Results：The accuracy rate of classification samples was 93% with 25 different gene,the accuracy rate was 87%-100% with 11 functional modules.Conclusion：These features function modules had some relevance to the occurrence of deficient cold syndrome.It was more favorable interpretation of the disease by studying functional modules based on gene expression profiles.

关 键 词：基因表达谱 基因本体 功能模块 特征基因 

分 类 号：R241[医药卫生—中医诊断学]