TRAIL联合多西紫杉醇对人鼻咽癌CNE2细胞的体外作用研究  

Synergistic Interactions of TRAIL and Docetaxel on the Nasopharyngeal Carcinoma Cell line in vitro

作　　者：姚鸿超[1] 肖辉[1] 李茗华[1] 王超[1] 刘鸣[1] 

机构地区：[1]黑龙江省哈尔滨医科大学附属第二医院耳鼻咽喉-头颈外科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2010年第6期1058-1060,共3页Progress in Modern Biomedicine

基　　金：教育部春晖计划项目(Z2008-1-15028);哈医大二院博士科研启动项目(BS2008-04)

摘　　要：目的:研究肿瘤坏死因子相关凋亡配体(TRAIL)联合化疗药物多西紫杉醇对鼻咽癌CNE2细胞凋亡诱导作用。方法:应用MTT法检测不同浓度多西紫杉醇的抗癌活性,计算其亚毒性剂量,流式细胞仪检测多西紫杉醇及TRAIL单独或者联合作用于鼻咽癌CNE2细胞后的细胞凋亡发生率,TUNEL法观察细胞凋亡发生情况。结果:鼻咽癌细胞对TRAIL的作用敏感,多西紫杉醇可以增强其凋亡诱导作用。结论:TRAIL与多西紫杉醇具有协同抗鼻咽癌作用,有望应用于鼻咽癌的临床治疗。Objective： To study the apoptosis inducing effect by Tumor necrosis factor related apoptosis-ligand （TRAIL）and doeetaxel on human nasophatyngeal carcinoma CNE2 cells. Methods： The human nasopharyngeal carcinoma CNE2 cells line was treated with different concentration of TRAIL and docetaxel in vitro. The inhibition ratio of tumor cells was determined by MTT colorimetric assay, the incidence of cell apoptosis was determined by flow cytometry method. The apoptosis inducing effect detemined by TUNEL. Resuits： Nasopharyngeal carcinoma CNE2 cells were resistant to apoptosis induced by TRAIL. Docetaxel could enhance its sensitivity to TRAIL by up-regulating the expressions of TRAIL death receptors in Hep-2 cells. Conclusion： TRAIL and doeetaxel have synergistric killing effects on nasopharyngeai carcinoma CNE2 cells, which has a promising prospect in the treatment ofnasopharyngeal carcinoma.

关 键 词：鼻咽癌 多西紫杉醇 凋亡 肿瘤坏死因子相关凋亡诱导配体 

分 类 号：R739.6[医药卫生—肿瘤]