大鼠脑缺血再灌注后Cathepsin D和caspase-9表达的动态变化  

Changes of CathepsinD and caspase-9 Expression in Rats after Cerebral Ischemia-Reperfusion

作　　者：张智博 刘柳青[2] 龚晓燕[2] 彭旭 陈本阳[2] 

机构地区：[1]长沙市第一医院神经内科,湖南长沙410005 [2]南华大学,湖南衡阳421001

出　　处：《现代生物医学进展》2010年第5期851-854,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨大鼠缺血再灌注后溶酶体组织蛋白酶D(Cathepsin D CD)、caspase-9在不同时段蛋白质及mRNA表达变化。方法:将60只S D大鼠随机分为三组:正常组(10只),假手术组(10只),脑缺血再灌注组(40只),线栓法制备大脑中动脉梗死模型(MCAO),免疫组化及RT-PCR法分别检测Cathepsin D、caspase-9的蛋白和mRNA表达。结果:与正常组和假手术组比较,模型组大鼠脑缺血再灌注损伤后6h Cathepsin D的蛋白和mRNA表达明显增强(P<0.05),24h达高峰,48h仍保存高水平。caspase-9蛋白和mRNA6h开始明显升高,12h达高峰,此后缓慢下降,但48h组仍显著高于对照组(P<0.05),结论:Cathepsin D、caspase-9在大鼠脑缺血再灌注后表达增强,溶酶体可能参与了脑缺血再灌注损伤后神经细胞凋亡的过程。Objective：To study the changes of the protein and mRNA of Cathepsin D and caspase-9 expression in different time point in rat after cerebral ischemia-reperfusion. Methods：60 SD rats were randomly divided into three groups： normal group （n=10）, sham-operation group （n=10）, cerebral isehemia-reperfusion group （n=40）, prepared by suture middle cerebral artery infarction model （MCAO）, the expression of the protein and mRNA of Cathepsin D and caspase-9were respectively evaluated by methods of immunohistoehemistry and RT-PCR. Results：compared with normal group and sham-operated group , The expression level of the protein and mRNA of Cathepsin D and easpase-9 in the rats of model group increased significantly 6 hour ,the formers got to their peak 24h while the latters got to their peak 12h ,both of them kept a high level on 48h. Conclusions ：The expression of Cathepsin D and caspase-9 after ischemia-reperfusion is high, Lysosomes may be involved in the process of neuronal apoptosis in the brain after ischemia-reperfusion injury

关 键 词：脑缺血再灌注 溶酶体 CATHEPSIND CASPASE-9 

分 类 号：Q95-3[生物学—动物学] R743[医药卫生—神经病学与精神病学]