兔玻璃体腔注射Dn-MyD88腺病毒载体观察对PVR形态学的影响  

The Morphology in Proliferative vitreoretinopathy after vitrectomy with vitreous injection of Dn-MyD88 Adenovirus Vector

作　　者：贾媛媛[1,2] 高晓唯[2] 田艳明[2] 任兵[2] 

机构地区：[1]石河子大学医学院新疆,石河子832000 [2]解放军474医院全军眼科中心,新疆乌鲁木齐830013

出　　处：《现代生物医学进展》2010年第5期861-864,874,共5页Progress in Modern Biomedicine

基　　金：兰州军区医药卫生科研基金项目资助(LXH-2006016)

摘　　要：目的:重组Dn-MyD88(Dominant negative myeloid differentiation factor88,Dn-MyD88)腺病毒载体观察对增殖性玻璃体视网膜病变形态学的影响。方法:向兔眼内注射富含血小板浓度为5×107～10×107/ml的血浆制作PVR模型,并对MyD88介导的NF-κB信号通路进行了分析。向兔眼玻璃体腔内转染腺病毒携带的无功能MyD88,运用眼B超,眼底照相等方法,于1,7,14,21,28天观察PVR形态学变化。结果:在增殖性玻璃体视网膜病变中通过转染腺病毒携带的无功能MyD88,与对照组相比发现增殖膜有减少趋势。结论:在兔眼PVR模型中转染腺病毒携带的无功能MyD88可抑制PVR的发生发展,可为临床PVR的治疗提供有益的参考和借鉴。Objective：To observe morphology of Proliferative vitreoretinopathy after injecting recombinat Dn-MyD88 adenovirus vector in rabbit. Methods： Inject the blood plasma including the blood platelet density for 5 × 10^7-10 ×10^7/ml to the rabbit＇s eyes to make the PVR model. Dominant negative myeloid differentiation protein （Dn--MyD88） fragment was inserted into adenovirus and then observing its appearance in 1,7,14,21,28 days. Results： Proliferative membrane reduced with the experiment group. Conclusion： MyD88-dependent NF-κB signaling is a novel pathway for inducing the development of PVR and blocking MyD88 mediated signaling pathway attenuates the development of PVR.

关 键 词：髓样分化蛋白 增殖陛玻璃体视网膜病变 基因治疗 

分 类 号：Q95-3[生物学—动物学] R774.1[医药卫生—眼科]