依达拉奉对大鼠缺血脑组织NGF及BDNF表达的影响  

The Effect of Edaravone on Expression of NGF and BDNF in Ischemia Stroke Tissue of Rat Cerebrum

作　　者：王炜[1] 张生林[1] 

机构地区：[1]山西医科大学第一临床医院老年病科,太原030001

出　　处：《中国医疗前沿》2010年第7期23-24,2,共3页China Healthcare Innovation

摘　　要：目的研究依达拉奉对大鼠缺血脑组织中脑源性神经生长因子(BDNF)和神经生长因子(NGF)表达的影响。方法健康雄性Wistar大鼠,采用改良的Zea-Longa法建立大脑中动脉局灶缺血模型(MCAO)。实验分为模型对照组和依达拉奉组,每组各20只大鼠。依达拉奉组予以腹腔内注射依达拉奉,1次/d,每次3mg/kg;模型对照组每天注射等量生理盐水。72h后行神经功能评分,然后处死,分别取脑梗死周围、海马区和梗死区脑组织作免疫组化染色检测BDNF、NGF蛋白表达变化,原位杂交检测BDNFmRNA、NGFmRNA的表达。结果依达拉奉组神经功能缺损评分低于模型对照组(p<0.05)。依达拉奉治疗组BDNF和NGF蛋白BDNFmRNA和NGFmRNA在梗死区周围和海马区表达均高于模型对照组,差异有统计学意义(p<0.05),在梗死区差异均无统计学意义(p>0.05)。结论依达拉奉可上调大鼠梗死区周围和海马区缺血脑组织中BDNF和NGF的表达,并可能通过此机制起到保护作用。Objective To study the effects of Edaravone on the protein and mRNA expression of brain derived neurotrophic factor（BDNF） and nerve growth factor（NGF） in the permanent focal cerebral ischemia tissues of rats.Methods Healthy male Wister rats were taken for this study project.According to the procedure of Zea-Longa,the rat model with permanent cerebral ischemia was established by rat middle cerebral artery obstructed（MCAO） with a nylon thread.Operated rats were randomly divided into model control and edaravone treated groups（n=20 for each）.By intraperitoneal injection,model control group rats were given physiological saline 3mg/kg once daily for 3days,and also edaravone treated group rats were given edaravone 3mg/kg once daily for 3days.5 rats in each group were taken to tetrazolium chloride（TTC） staining for macroscopic observation of cerebral infarction area,the rest samples were processed by immunohistochemistry to evaluate effects of edaravone on BDNF and NGF expression,hybridization in situ to evaluate effects of edaravone on BDNF mRNA and NGF mRNA expression.SPSS12.0 for statistical analysis,it was p〈 0.05 as having statistical significance.Results Comparing to model control group,edaravone treated group did not have significantly pathological difference,but the grade of behavior and infarction area were apparently reduced（p 〈0.05）.In edaravone group,there was a significant expression up-regulation to BDNF,NGF,BDNF mRNA and NGF mRNA in the peripheral around infarction and cornu ammonis or hippocampus area（p〈 0.05）.However in the infarction area,the expressions of BDNF,NGF,BDNF mRNA and NGF mRNA had no significantly statistical difference（p 〉0.05）.Conclusion Comparing to control group,edaravone can significantly up-regulate the expressions of BDNF and NGF in genetic transcription level,and protect from the ischemia injury.

关 键 词：依达拉奉 缺血性脑血管病 神经生长因子(NGF) 脑源性神经生长因子(BDNF) 

分 类 号：R743.33[医药卫生—神经病学与精神病学]