原位杂交检测乙肝病毒携带者精子中乙肝病毒-DNA的研究  

Detection of hepatitis B virus-DNA of spermatozoa samples from hepatitis B virus carrier by in situ hybridization

作　　者：梅顺利[1,2] 刘平[1] 张小为[1] 黄锦[1] 张秋芳[1] 张莹[1] 

机构地区：[1]北京大学第三医院妇产科,北京100191 [2]北京市第二医院妇产科,北京100031

出　　处：《生殖医学杂志》2010年第2期131-133,共3页Journal of Reproductive Medicine

摘　　要：目的通过检测乙肝病毒携带者经辅助生育技术洗涤后的精子是否携带有乙型肝炎病毒,从而判断是否存在经精子传播乙型肝炎的可能性。方法用地高辛标记,针对乙型肝炎病毒DNA(HBV-DNA)设计的探针,对来我院要求行辅助生育治疗的20例乙肝病毒携带者的精子,经洗涤离心上游后进行检测。结果发现8例携带者精子中有乙肝病毒DNA,分布于精子的头部,阳性精子计数占每片精子总数的3‰～10‰。结论经辅助生育技术洗涤后的乙肝病毒携带者的精子仍可传播HBV-DNA。Objective： To detect whether the washed spermatozoa in assisted hepatitis B virus carrier carry hepatitis B virus-DNA （HBV-DNA）. Methods. The washed spermatozoa samples were got from twenty （HBsAg ＋, HBeAg ＋, HBcAb ＋） and made smears. These samples tion using Digoxin-labeled HBV-DNA probe. reproductive techniques from the males of hepatitis B virus carrier were detected by in situ hybridizaResults： Eight spermatozoa smears were HBV-DNA positive, with the positive signals located at the spermatozoa heads. Conclusions： HBV-DNA exists in the sperm of the hepatitis B virus carrier. So there is a possibility that HBV infection could be transmitted by the infected reproductive cells.

关 键 词：精子 乙型肝炎病毒 原位杂交 

分 类 号：R[医药卫生]