慢性粒细胞白血病Ph染色体和bcr-abl mRNA定量检测的临床意义  

Clinical significance of detection of Ph chromosome and Bcr-abl mRNA in chronic myeloid leukemia

作　　者：吕晓东[1] 范瑞华[1] 胡杰英[1] 徐本玲[1] 张旭华[1] 郭金东[1] 宋云峰[1] 宋永平[1] 

机构地区：[1]河南省肿瘤医院中心实验室,河南郑州450008

出　　处：《西安交通大学学报（医学版）》2010年第3期325-327,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：河南省社会公益项目预研专项课题(No.072103810503)~~

摘　　要：目的探讨Ph染色体和bcr-abl mRNA在慢性粒细胞白血病诊断、分期、治疗及微小残留病变监测中的意义。方法同时检测437份慢粒标本的Ph染色体和bcr-abl mRNA的表达情况。结果慢性期、加速期和急变期的bcr-abl mRNA定量结果逐渐增高(P<0.05);异基因造血干细胞移植后的bcr-abl mRNA定量结果随时间逐渐降低,一般在移植6月后转阴,服用格列卫与异基因造血干细胞移植病程相似,但融合基因转阴所用时间较长。结论实时定量PCR技术检测bcr-abl mRNA较Ph染色体的检测更为敏感,对于疾病分期、评价疗效、监测微小残留病变以及预测疾病进展具有重要的临床应用价值。Objective To evaluate the significance of Ph chromosome and bcr-abl mRNA expressions in diagnosis,staging and treatment of chronic myeloid leukemia（CML） as well as in monitoring minimal residual disease.Methods Expressions of Ph chromosome and bcr-abl mRNA of 437 CML patients were detected at the same time.Results Expression of bcr-abl mRNA gradually increased in blastic phase（BP）,accelerated phase（AP） and chronic phase（CP）（P〈0.05）.Quantification of bcr-abl mRNA decreased gradually after allotransplantation in the patients,and it became normal after 6 months＇ treatment.However,quantification of bcr-abl mRNA in imatinib mesylate-treated patients did not become normal until longer time later.Conclusion RT-PCR quantitative detection of bcr-abl mRNA is more sensitive than detection of Ph chromosome,and has an important clinical value in staging CML,monitoring treatment outcome,detecting the minimal residual disease and predicting disease development.

关 键 词：慢性粒细胞白血病 实时定量PCR PH染色体 BCR-ABL 

分 类 号：R733.72[医药卫生—肿瘤]