RAPD和SRAP分子标记在绣球菌菌种鉴定中的应用  

Identification the Relationship of Sparassis crispa Strains Using RAPD and SRAP Molecular Markers

作　　者：陈瑞鹏[1] 贾小宁[1] 郭立忠[1] 

机构地区：[1]青岛农业大学生命科学学院山东省应用真菌重点实验室,山东青岛266109

出　　处：《中国食用菌》2010年第2期34-36,共3页Edible Fungi of China

基　　金：国家支撑项目;黄淮平原区农田秸秆资源综合利用技术集成研究与示范(2007BAD89B09-15)

摘　　要：采用RAPD、SRAP两种标记方法分别对6个绣球菌(Sparassis crispa)菌株进行亲缘关系分析,结果分析表明2种方法结果一致,平均每条引物组合产生的条带数分别为20.5和24.5,多态性条带的比例分别为91.4%和78.8%。结果显示,6个菌株间均有遗传上的差异,菌株4号、5号同源性最大,相似系数分别达到了0.981和0.875,说明菌株4号、5号可能是同物异名。Six strains of Sparassis crispa conserved by Agricultural Applied Mycology Lab, Qingdao Agricultural University, were studied by using the molecular techniques of RAPD and SRAP in this experiment. The results showed that the results of two methods are consistent. Each primer combination generated the average strip of 20.5 and 24.5, respectively. The proportion of polymorphic bands were 91.4% and 78.8% respectively, and 6 strains were different at genetic. Sparassis crispa strain 4 and 5 exhibited a higher genetic similarity （similar coefficients were up to 0.9813 and 0.8570）, which showed close phylogenetic relationship.

关 键 词：绣球菌 RAPD SRAP 聚类分析 

分 类 号：S646.9[农业科学—蔬菜学]