5个野生木耳属菌株的ITS PCR-RFLP分析  

Analysis of 5 Wild Auricularia Strains with ITS PCR-RFLP Markers

作　　者：贾定洪[1] 郑林用[1] 郭勇[1] 王波[1] 甘炳成[1] 黄忠乾[1] 彭卫红[1] 

机构地区：[1]四川省农业科学院土壤肥料研究所,四川成都610066

出　　处：《西南农业学报》2010年第2期449-453,共5页Southwest China Journal of Agricultural Sciences

基　　金：四川省财政育种工程资助项目"食用菌新品种选育及新材料新方法研究"(2006YZCC09-024);四川省育种攻关资助项目"优质丰产黑木耳新品种选育"(YZGG06-04-01)的部分研究内容

摘　　要：采用ITSPCR-RFIt方法对5个野生木耳属菌株进行分析，并以7个黑木耳栽培菌株和1个毛木耳菌株为参照。通过6个限制性内切酶消化共获得20条清晰易辨的多态性DNA条带，使用NTSYspe2．1生物软件对DNA条带信息进行分析，结果显示：13个木耳属菌株的遗传相似系数变异范围为0．500—1．000，遗传相似系数变化较大；13个供试菌株在相似系数为0．547时聚在一起，在0．721时聚为3个类群；实验结果支持Auhm、Au5931和Au59641为黑木耳属菌株；Audx菌株鉴定为毛木耳属菌株。研究结果表明ITSPCR—RFLP技术可为区别黑木耳和毛木耳菌株提供重要信息。5 wild Auricularia strains were studied using internal transcribed spacer （ITS） PCR-RFLP method with 7 cultivated Auricularia auricula and 1 Aur/cu/ar/a polyrricha strains as control. Results showed there were,in total ,20 distinct and polymorphic DNA bands after di- gested by 6 restriction endonucleases respectively. The dendregram was generated by unweighted pairgroup method with arithmetic average （UPGMA） method with NTSYSpc2.1 bio-software. Results showed： （i） The similarity coefficients of 13 Auricuhtria Strains ranged from 0. 108 to 0. 822; （ii） All strains were clustered together at the similarity coefficient of 0. 547 ,and clustered into 3 groups at the similarity coefficient of 0.721 ; （ iii ） Accessions of Auhm, Au5931 and Au59641 were considered as Auricularia auricular strains, while accession of Audx was identified as Auricuiarla polytricha strain. And ITS PCR-RFLP technique proved a vital role to play in distinguishing between Auricuiarla auricular and Auricularia polytricha strains.

关 键 词：黑木耳 毛木耳 遗传鉴定 

分 类 号：S646.6[农业科学—蔬菜学]