CD105和血管内皮生长因子在大鼠角膜新生血管中的表达  

The Expression of CD105 and VEGF in Corneal Neovascularization of Rats

作　　者：丁怡[1] 张明昌[1] 

机构地区：[1]广州军区武汉总医院眼科,武汉430070

出　　处：《华中科技大学学报（医学版）》2010年第2期221-224,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的研究CD105在大鼠角膜新生血管（CNV）中的表达,并研究其和血管内皮生长因子（VEGF）的相关性。方法用含有100 ng/μL鼠碱性成纤维细胞生长因子（bFGF）1μL的缓释药丸放入36只SD大鼠角膜囊袋内,建立大鼠角膜新生血管模型;按照不同时间点,对角膜新生血管的情况进行形态学分析,用免疫组织化学和计算机图像分析系统检测新生血管形成不同时间点CD105和VEGF在角膜中的表达。结果在模型制作后第4天开始长出新生血管,第7~10天达高峰,3周以后开始明显减少。免疫组化结果显示CD105和VEGF的表达情况与新生血管的生长呈平行关系。结论CD105与角膜新生血管形成有相关性,在新生血管生长中扮演着重要角色。Objective To study the expression of CD105 in corneal neovascularization（CNV） of rats,and its relation with vascular endothelial growth factor（VEGF）.Methods The pills containing 100 ng/μL rat-derived basic fibroblast growth factor（b-FGF） 1 μL were transplanted into the corneal pockets of 36 rats to establish the rat CNV models.At the indicated time points,morphologic changes in CNV were observed,and the expression of CD105 and VEGF was detected by immunohistochemistry and computer image analysis.Results The CNV began to grow on the 4th day,reached the peak on the 10th day,and reduced after 3 weeks.The expression of CD105 and VEGF was parallel to the growth of CNV.Conclusion CD105 has a relation to the CNV and plays a necessary role in the growth of CNV.

关 键 词：CD105 血管内皮生长因子 角膜新生血管 

分 类 号：R772.2[医药卫生—眼科]