冬虫夏草HPCE指纹图谱测定中样品的预处理方法  

Exploration of different pretreatment methods for cordyceps sinensis by HPCE

作　　者：阎正[1] 张智慧[1] 于趁[1] 蔡立鹏[1] 苑若瑶[1] 王春云[1] 王继坤 

机构地区：[1]河北大学化学与环境科学学院,河北保定071002 [2]北京中防昊通防伪技术中心,北京100025

出　　处：《医学研究与教育》2010年第2期4-6,共3页Medical Research and Education

基　　金：国家科技支撑项目(2006BAK02A17)

摘　　要：目的探索冬虫夏草不同的预处理方法,以便更好地反映冬虫夏草的谱图信息。方法采用不同的预处理方法,比较操作方法和所得样品溶液。结果通过比较发现,在以下电泳条件及改进索氏提取法所得谱图峰形好,信息多。电泳条件:未涂渍弹性石英毛细管(75μm×60cm);缓冲溶液:40mmol/L硼砂溶液;电泳电压:20kV;进样时间:3s;电泳温度:室温;检测波长:250nm。结论通过多种预处理方法的比较,上述预处理方法操作简便、所得溶液澄清透明。Objective To describe some different sample pretreatment methods to express fingerprint of cordyceps completely. Methods Different pretreatment methods were applied to compare operation methods and sample solution. Results Under the following conditions and with improved Soxhlet Extraction,we could get a fingerprint with better peak shape and more information compared with others, a 75 μm×60 cm uncoated capillary was used with 40 mmol/L borate as CE buffer;the run voltage was 20kV; the injecting time was 3 s and the temperature was 25 ℃, the detection wavelength was 250 nm. Conclusion The pretreatment method is simple and the solution is clear.

关 键 词：预处理方法 冬虫夏草 高效毛细管电泳 

分 类 号：O657.7[理学—分析化学]