卵磷脂改性对壳聚糖微球及缓释性能的影响  

Preparation of chitosan-phospholipid complex microspheres and its ability of drugs-releasing

作　　者：武金霞[1] 李瑞[1] 刘雪英[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《医学研究与教育》2010年第2期7-10,共4页Medical Research and Education

基　　金：河北大学博士基金项目(y2006089)

摘　　要：目的研究卵磷脂对微球的载药能力以及对药物缓释的影响。方法以BSA作为模式药物,采用超声乳化法制作微球,分别用微球吸附和包埋BSA。将载药微球分别进行体外释放、人工胃液和人工肠液中的释放,每隔一段时间测定溶出的蛋白量。用聚丙烯酰胺凝胶电泳检测溶出的BSA稳定性。结果超声乳化法制作的两种微球中,卵磷脂改性壳聚糖微球包埋BSA和吸附BSA载药率分别为55.1%和22.0%,包埋率分别为61.8%和33.7%。壳聚糖微球包埋BSA和吸附BSA载药率分别为44.8%和23.9%,包埋率分别为50.6%和32.8%。载药微球在体外释放的释放率均小于75%,在人工肠液中的释放率高于人工胃液中的释放率。聚丙烯酰胺凝胶电泳能检测到完整的BSA条带。结论卵磷脂改性壳聚糖微球的载药率高,缓释效果好,模型药物BSA在制作微球过程中没有降解。Objective To evaluate the ability of drug-loadding and drug-sustained releasing of the chitosan and the chitosan-phospholipid complex microspheres. Methods The microspheres were prepared by ultrasound emulsification method. BSA was encapsulated and adsorbed into micropheres respectively as model drug. The microspheres were dipped in vitro and artifical gastroenteric environment separately, and the ability of the microspheres carrying drugs and the sustained releasing was detected by drug loaded rate, encapsulation efficiency and release rate. Results The loaded rate of chitosan-phospholipid complex microspheres made by ultrasound emulsification encapsulated BSA and absorbed BSA were 55.1% and 22.0% ,the encapsulation efficiency were 61.8% and 33.7% respectively, at the same time the loaded rate of chitosan microspheres encapsulated BSA and adsorbed BSA were 44.8% and 23.9% ,the encapsulation efficiency were 50.6% and 32.8% . The release rate of all the microspheres in vitro were all less than 75% . The release rate of microspheres in the artificial intestinal liquid was higher than that in the simulative gastric juice. SDS-PAGE of BSA released from microspheres showed the BSA band clearly. Conclusion The chitosan -phospholipid complex microspheres prepared by ultrasound emulsification possess the highest loaded rate and show best drug-sustained releasing behavior , the model drug BSA is not degraded during the preparation.

关 键 词：壳聚糖微球 超声乳化法 卵磷脂改性 包埋率 

分 类 号：R917[医药卫生—药物分析学]