油菜内生细菌Yc8生防作用的初步研究  

Preliminary Study on Bio-control of Rape Endophytic Bacteria Yc8

作　　者：刘慧芹[1] 邢鲲[2] 王丽 刘慧平[2] 韩巨才[2] 

机构地区：[1]天津农学院园艺系,天津300384 [2]山西农业大学农学院,山西太谷030801 [3]天津动物园,天津300381

出　　处：《北方园艺》2010年第9期169-171,共3页Northern Horticulture

基　　金：山西省留学归国基金资助项目(2007061;2009043);山西省科技攻关资助项目(2007031039)

摘　　要：从健康油菜植株中分离并筛选出1株内生细菌Yc8,现对其生防作用进行了初步研究。结果表明:Yc8发酵液对15种病原菌中10种具有抑制作用,并且对油菜菌核病菌、稻瘟病菌的作用明显,抑制率可达80%;离心得到的发酵液对15种病原菌的抑制率要高于高温高压获得的发酵液的抑制率,表明Yc8对高温高压比较敏感;Yc8发酵液可使油菜菌核病菌发生菌丝肿胀,细胞壁破裂、细胞畸形等现象;油菜菌核病菌引起的植物离体组织腐烂试验中,Yc8发酵液表现出一定的防治作用,其保护作用大于治疗作用。Biocontrol of endophytic bacteria Yc8 isolated from the body of healthy rape was reliminary study. Results showed Yc8 fermenting solution had inhibiting effect to 10 pathogens in 15 pathogens. Inhibiting effect of it to Sclerotinia sclerotiorum and Magnaporthe grisea was obvious, and the inhibiting rate was up to 80 %. Fermenting solution of centrifugal had higher inhibiting rate to 15 pathogens than one of high temperature-pressure, which indicated it was more sensitive to high temperature-pressure. Yc8 fermenting solution made mycelia of S. sclerotiorum swelle, cell walls break down, cell occurred deformity. When S. sclerotiorum caused plant tissue（in vitro） rot, Yc8 fermenting solution showed some control effect, and protection was greater than therapy.

关 键 词：油菜内生细菌 发酵液 抑制作用 生防作用 

分 类 号：S643.3[农业科学—蔬菜学]