实时荧光定量PCR检测人肺腺癌细胞Anip973、Anip973/NVB中hTERT mRNA的表达  

Expression of hTERT mRNA in Anip973 and Anip973/NVB Cell Detected by Quantitative Real-time PCR

作　　者：陈公琰[1] 郭淑英 柳菁菁[3] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院内一科,哈尔滨150040 [2]广东省深圳市西丽人民医院,深圳518055 [3]吉林省肿瘤医院内一科,长春130021

出　　处：《中国肺癌杂志》2010年第4期297-300,共4页Chinese Journal of Lung Cancer

基　　金：教育部"春晖计划"合作科研项目(No.Z2006-1-15006)资助~~

摘　　要：背景与目的端粒酶逆转录酶为端粒酶的催化亚单位,其活性与细胞对化疗药物的敏感性密切相关。本实验研究人肺腺癌Anip973/NVB耐药细胞株与亲代细胞Anip973细胞株端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)的mRNA表达及差异,探讨端粒酶与耐药的相关性。方法采用实时荧光定量RT-PCR法检测人肺腺癌Anip973/NVB耐药细胞与Anip973亲代细胞经NVB处理前后hTERT mRNA的表达。结果对照组Anip973、Anip973/NVB细胞的hTERT mRNA表达无统计学差异;NVB处理组Anip973细胞hTERT mRNA表达明显降低(P<0.01),而Anip973/NVB细胞hTERT mRNA表达的降低与对照组比较无统计学差异(P>0.05),NVB处理组Anip973细胞hTERT mRNA表达降低的程度明显高于Anip973/NVB细胞(P<0.01)。结论端粒酶与肺腺癌耐药细胞的多药耐药密切相关,端粒酶有可能作为逆转耐药的新靶点。Background and objective Human telomerase reverse transcriptase is the catalytic subunit of telomerase,and its activity is correlated with cell’s sensitivity to chemotherapy.The aim of this study is to investigate the differential expression of human telomerase reverse transcriptase （hTERT） mRNA in human lung adenocarcinoma cell line Anip973 and Anip973/NVB,and to observe the correlation between hTERT mRNA and drug-resistance.Methods The real-time fluorescence quantitative RT-PCR was used to detect the change of hTERT mRNA in human lung adenocarcinoma drug-resistant cell Anip973/NVB and parental cell Anip973 treated by NVB.Results In the control group,the expression of hTERT mRNA showed no significant difference between drug-resistant cell Anip973/NVB and parental cell Anip973.After been treated by NVB,the expression of hTERT mRNA in parental cell was significantly decreased （P0.01）,and drug-resistant cell Anip973/ NVB had no evidently variant （P0.05）.The down-regulated hTERT mRNA in Anip973 cell was higher than that in Anip973/ NVB cell.Conclusion Telomerase correlates with the drug-resistant cell A973/NVB,and telomerase may be a new target for multi-drug resistant inversion.

关 键 词：肺腺癌 HTERT 长春瑞滨 实时荧光定量RT-PCR 多药耐药 

分 类 号：R734.2[医药卫生—肿瘤]