全基因组芯片筛查非小细胞肺癌组织多药耐药相关基因  

cDNA Microarray Technique on Screening Multi-drug Resistance-related Genes of Human Non-small Cell Lung Cancer

作　　者：刘美燕[1] 李春红[1] 闫安[1] 蔡莉[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院,哈尔滨150001

出　　处：《中国肺癌杂志》2010年第4期322-325,共4页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金项目(No.30772540);黑龙江省科委攻关课题(No.GC07C349);黑龙江省教育厅骨干教师项目(No.11519G026)资助~~

摘　　要：背景与目的筛查与非小细胞肺癌多药耐药相关的基因,为非小细胞肺癌的个体化治疗提供理论依据。方法将手术切除的肺癌组织细胞进行原代培养。首先,采用MTT法检测诺维本、吉西他滨、多西他赛、紫杉醇及顺铂对非小细胞肺癌组织细胞的抑制率和敏感性。再利用全基因组芯片筛选人高度敏感组和耐药组间的差异表达基因。结果共筛选出差异表达基因212个,与耐药组相比,高度敏感组中上调基因168个,下调基因44个。结论利用全基因组芯片筛查出212个可能与非小细胞肺癌多药耐药相关的基因,用于指导临床个体化治疗。Background and objective The aim of this study is to screen for multi-drug resistance-related genes of human non-small cell lung cancer （NSCLC）,and provide the evidences for drug-sensitive predicting genes of different NSCLC patients treated with chemotherapeutic drugs.Methods Sensitivity and inhibition ratio of five antitumor drugs （NVB,GEM,TAL,DOC,CDDP） on 75 fresh NSCLC samples from different individuals were studied by means of culturing primary tumor cells and MTT assay.After the five chemotherapeutic drugs were used,multi-drug resistance-related genes of NSCLC with cDNA microarry on the samples which were all high sensitive and those resistant were screened.Results cDNA microarray analysis screened out 212 genes,168 of which were up-regulated while the other 44 were down-regulated in the group of highly sensitive compared with the group of resistance.Conclusion The multi-drug resistance of NSCLC may be correlative with the 212 genes screened by cDNA microarray;the detailed mechanisms of the genes still need to be detected in the future.

关 键 词：肺肿瘤 MTT法 全基因组芯片 多药耐药 

分 类 号：R734.2[医药卫生—肿瘤]