重组人血管内皮抑制素联合放射治疗对lewis肺癌小鼠的作用  

Effects of Rh-endostar in Combination with Radiotherapy on Rats with Lung Cancer

作　　者：何渡[1] 戈伟[1] 李长虎[1] 赵振宇[1] 徐细明[1] 杨芳[2] 

机构地区：[1]武汉大学人民医院肿瘤科,武汉430060 [2]武汉大学基础医学院,武汉430060

出　　处：《中国肺癌杂志》2010年第4期386-390,共5页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金(No.30970860)项目资助~~

摘　　要：背景与目的放疗敏感性与组织中氧含量关系密切,单用重组人血管内皮抑素(rh-endostatin,YH-16,恩度)可能通过血管"正常化"作用使组织中氧含量增加,这与放疗的敏感性密切相关。本研究将重组人血管内皮抑素与放射治疗联合对lewis肺癌小鼠的肿瘤生长及血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法制作lewis肺癌细胞种植肿瘤的动物模型,小鼠随机分为四组:A组为空白对照组,B组为重组人血管内皮抑素组,C组为放疗组,D组为联合组。分别给予相应处理后绘制肿瘤生长曲线,计算抑瘤率,用免疫组化(SP法)测定VEGF的表达及微血管密度。结果经过相应的处理后,各处理组较A组移植瘤的生长速度明显减慢。且各组瘤质量明显低于A组(P<0.05),D组较其它三组降低得更加明显(P<0.05),VEGF的表达及微血管密度与A组相比也有不同程度的降低(P<0.05),其中D组下降最为明显。结论重组人血管内皮抑素和放疗联用对lewis肺癌小鼠有明显肿瘤抑制作用,其机制可能是下调VEGF的表达、抑制新生血管的生成。Background and objective Radiation sensitivity is closely related to tissue oxygen,and rh-endostatin can induce the high level of oxygen content in tumor by ＂normalizing＂ tumor angiogenesis which is associated with radiotherapy sensitivity.The aim of this study is to observe the effect of combination of radiotherapy with rh-endostatin in the rats with lung cancer.Methods Immediate lewis cancerous ascetic injection method was used to make rats tumors bearing model,then the rats was divided into four groups randomly： group A was treated with saline;group B was treated with rh-endostatin;group C was treated with irradiation and group D was treated with rh-endostatin and irradiation.After all rats were treated,inhibition rates and the tumor growth curve were calculated.Immunohistochemisty was adopted to check the expressions of vascular endothelial growth factor （VEGF） and microvessel density （MVD）.Results Compared with group A,the growth rates of the tumors in the other group were obviously slower,and the tumor weights were significantly different form group A （P0.05）.Compared with the other groups,the tumor weights of group D were obviously reduced （P0.05）.Compared with group A,VEGF and MVD of other three groups were reduced （P0.05）,and group D were significantly cut down.Conclusion Combination with radiotherapy and rh-endostatin could inhibit the lung cancer significantly in rats.The possible mechanisms are to decrease the expression of VEGF and inhibit the production of angiogenesis.

关 键 词：肺肿瘤 放射治疗 重组人血管内皮抑制素 

分 类 号：R734.2[医药卫生—肿瘤]