雌激素受体基因多态性与慢性牙周炎相关性研究  

Estrogen receptors gene polymorphisms in patients with chronic periodontitis

作　　者：张璇[1,2] 龙铟[3] 李红燕[4] 李晓娟[5] 丁寅 

机构地区：[1]第四军医大学口腔医院正畸科,陕西西安710032 [2]中国人民解放军,总医院口腔科,北京100853 [3]第四军医大学西京医院中医科,陕西西安710032 [4]天水市第一人民医院口腔科,甘肃天水741000 [5]第四军医大学西京医院骨密度研究室,陕西西安710032

出　　处：《华西口腔医学杂志》2010年第2期139-144,共6页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目(30572069)

摘　　要：目的探讨雌激素受体基因多态性与陕西地区慢性牙周炎易感性的关联。方法收集陕西地区109例慢性牙周炎患者和99例牙周健康对照组的颊黏膜拭子,用Chelex-100方法提取全基因组DNA,用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测雌激素α和β受体基因型分布。结果慢性牙周炎组与正常对照组在ER-α受体XbaⅠ基因型分布上有统计学差异,慢性牙周炎组XX型基因频率明显高于正常对照组,尤其在女性患者中此差异显著,男性患者中未见不同;ER-β受体RsaⅠ和AluⅠ基因型在患者组与对照组中分布未见差异。结论慢性牙周炎易感性与雌激素XbaⅠ基因型分布相关,汉族女性群体中ER-α受体XX基因型可能为慢性牙周炎的易感因子。Objective To determine whether polymorphisms in the estrogen receptor（ER） genes are associated with chronic periodontitis in a Chinese population overall the Shanxi territory. Methods 109 patients with chronic periodontitis and 99 healthy controls were recruited in this study. Genomic DNA was extracted from oral mucosa swab sample of each subject by the Chelex-100 method. Determination of the ER-α and -β polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）, respectively. Results The analysis of the XbaⅠ polymorphisms in ER-α gene revealed significant differences between patients and controls. The detection frequency of XX genotype was significantly higher in the chronic periodontitis patients than in control subjects. The difference between the female chronic periodontitis patients and healthy controls was statistically significant, but no difference was found between the male patients and controls. Nevertheless, no significant association was noted in the frequency of both RsaⅠ and AluⅠ polymorphisms in ER-β gene between chronic periodontitis and controls. Conclusion It is indicated that the ER-α XX genotype may be a susceptible indicator for chronic periodontitis in female Han Chinese subjects.

关 键 词：雌激素Α受体 雌激素Β受体 基因多态性 慢性牙周炎 

分 类 号：R781.4[医药卫生—口腔医学]