成人正畸患者龈下菌斑中牙龈卟啉单胞菌的变化  

Quantitative study of Porphyromonas gingivalis in subgingival plaques of orthodontic adults

作　　者：陆卉[1] 周慧敏[2] 宋晓波 孙境庐 刘红彦[1] 

机构地区：[1]大连医科大学附属第一医院口腔正畸科,辽宁大连116011 [2]大连医科大学微生物教研室,辽宁大连116000 [3]特隆姆瑟大学医学微生物系

出　　处：《华西口腔医学杂志》2010年第2期166-169,共4页West China Journal of Stomatology

基　　金：辽宁省科委自然科学基金资助项目(20062153)

摘　　要：目的研究成人正畸患者在固定矫治器戴入后,其牙周临床指标和龈下菌斑中牙龈卟啉单胞菌(P.gingivalis)的早期变化。方法选择成人正畸患者11例,分别在矫治器戴入前,戴入后第1、3个月检查牙周临床指标(包括菌斑指数、龈沟出血指数、探诊深度和附着丧失),同时采集龈下菌斑样本,利用荧光实时定量聚合酶链反应检测样本中P.gingivalis和总细菌的数量,计算出P.gingivalis的检出率和构成比。分析牙周临床指标和P.gingivalis的检出率、构成比在不同观察时点的变化情况。结果菌斑指数、龈沟出血指数在矫治器戴入后均比戴入前明显升高(P<0.05)。探诊深度在矫治器戴入1个月后升高(P<0.05),3个月后下降至基线水平。试验中未发现有探诊深度大于2mm的患者,也未发现有附着丧失的患者。在3次检测中,P.gingivalis检出率均为45.5%,而P.gingivalis构成比的变化也无统计学差异(P>0.05)。结论固定矫治器戴入早期可引发成人正畸患者口内局部菌斑堆积,菌群中P.gingivalis增殖,出现轻度牙龈炎。Objective To assess the early variation of clinical periodontal indexes and Porphyromonas gingivalis （P.gingivalis） in subgingival plaques of orthodontic adults. Methods 11 orthodontic adults were selected. Clinical periodontal indexes of observed teeth were examined at three different time points： Before orthodontic treatment, the first month after treatment and the third month after treatment, and subgingival plaques were collected simultaneously at each time point. Clinical periodontal indexes included four ones： Plaque index（PLI）, sulcus bleeding index（SBI）, probing depth（PD）, and attachment loss. We used real -time quantitative polymerase chain reaction to detect and quantitate the number of total bacteria and P.gingivalis in each sample to obtain positive rate of P.gingivalis and the percentage of P.gingivalis in total bacteria. Results PLI and SBI of the first and third month were more than that of the baseline（P0.05）. PD rose at the first month after treatmen（tP0.05）, and then dropped at the third month. PD of all the 11 participants was lower than 2 mm. No attachment loss was found. The positive rate of P.gingivalis was stable（45.5%） and the proportion of P.gingivalis had no significant difference（P0.05） at each time point. Conclusion Fixed orthodontic appliances caused plaque accumulation, accordingly slight gingiva inflammation and the increasement of P.gingivalis occurred in the early stage, but none periodontitis was found.

关 键 词：固定矫治 牙龈卟啉单胞菌 实时定量聚合酶链反应 牙周临床指标 

分 类 号：R783.5[医药卫生—口腔医学]