去甲泽拉木醛对T24膀胱癌细胞株药效强度的实验研究  

Study on Efficacy Strength of Demethylzeylasteral on T24 Bladder Cancer Cell Lines

作　　者：尹瀚堃[1] 许乐[1] 朱煜[1] 杨春欣[1] 林宗明[1] 

机构地区：[1]复旦大学附属中山医院泌尿外科,上海200032

出　　处：《中国临床医学》2010年第2期228-230,共3页Chinese Journal of Clinical Medicine

基　　金：国家科技部重大科研基金项目(编号:2009ZX09301-011)

摘　　要：目的:探讨去甲泽拉木醛对T24膀胱癌细胞株作用的浓度-效应关系及时间-效应关系。并以吡柔比星为对照,研究两者对膀胱癌细胞株的杀伤作用有无差异。方法:分别用两个不同浓度的去甲泽拉木醛(20μg·mL-1和40μg·mL-1)和吡柔比星(0.03μg·mL-1和0.06μg·mL-1)处理T24膀胱癌细胞株,连续用3d后用MTT法测定细胞活性,计算抑制率。结果:去甲泽拉木醛组和吡柔比星组对T24膀胱癌细胞的杀伤率随时间的延长而增高。两个浓度组的去甲泽拉木醛和吡柔比星对T24膀胱癌细胞的杀伤率的差异在第3天有统计学意义(低浓度组:0.624±0.035比0.563±0.032,P=0.015;高浓度组:0.792±0.026比0.672±0.028,P<0.001)。在第1天和第2天,两个浓度组的去甲泽拉木醛和吡柔比星的杀伤率差异无统计学意义。(低浓度组:第1天0.152±0.063比0.127±0.055,P=0.456;第2天0.504±0.031比0.507±0.042,P=0.879。高浓度组:第1天0.266±0.068比0.231±0.057,P=0.256;第2天0.585±0.053比0.594±0.039,P=0.660)。结论:去甲泽拉木醛对T24膀胱癌细胞株有明确而强烈的抑制与杀伤作用,并在作用的第1天和第2天,其杀伤作用与相对应的浓度组吡柔比星近似。Objective：Investigate the dose-effect and time-effect connection of perarubicin （THP） and demethylzeylasteral to bladder cancer cell line T-24,and to compare the cytotoxicity of THP and demethylzeylasteral. Methods：Cytoactivity of T-24 cells were measured after treated with varies concentrations and index of cytotoxicity （IC） was calculated. Results：IC of THP and demethylzeylasteral were increased with the increase of treated time. There was a significant difference between IC of the lower and higher concentration group of THP and demethylzeylasteral on the third day （lower group：0.624±0.035 vs. 0.563±0.032,P= 0.015 ;higher group：0.792±0.026 vs. 0.672±0.028,P0.001 ）. There was no significant difference between IC of the lower and higher concentration group of THP and demethylzeylasteral on the first and second day （Lower group：the first day：0.152±0.063 vs. 0.127±0.055,P=0.456; the second day 0.504±0.031 vs. 0.507±0.042,P= 0.879. Higher group：the first day：0.266±0.068 vs. 0.231±0.057,P=0.256; the second day 0.585±0.053 vs. 0.594±0.039,P= 0.660）. Conclusions：Both the lower and higher concentration groups of THP and demethylzeylasteral have a similar cytotoxicity to bladder cancer cell line T-24 in the first day and second day.

关 键 词：去甲泽拉木醛 膀胱癌 体外试验 

分 类 号：R737.14[医药卫生—肿瘤]