测定烟叶糖苷配基香味成分的酶水解条件优化研究  

Study on the optimization for determination of aglycones of glycosides in tobacco by enzymic hydrolysis

作　　者：谢明利[1] 牟定荣 毛多斌[1] 董伟 白晓莉 

机构地区：[1]郑州轻工业学院烟草科学与工程学院,河南郑州450002 [2]红塔集团有限责任公司技术中心,云南玉溪653100

出　　处：《郑州轻工业学院学报（自然科学版）》2010年第2期9-12,共4页Journal of Zhengzhou University of Light Industry:Natural Science

摘　　要：采用SDE/GC-MS法对源于杏仁的糖苷酶水解烟叶糖苷配基进行了分离和测定.确定测定烟叶糖苷配基香味成分的酶最佳水解条件为:温度38℃,时间48 h,料液比400 mL/30g烟叶,pH=5.0,酶添加量40 mg.在此条件下,烟叶香味成分萃取量可达179.06×10-4%.The separation and determination for aglycones of glycosides in tobacco which were hydrolyzed by glycosidase source from almond by SDE/GC-MS was researched. The result showed that the optimal hydrdysis conditions to analyze the aglycones of glycosides in tobacco were temperature 38 ℃, time 48 h, solvent and solid ratio 400 mL/30 g, pH = 5.0 and enzyme addition of aspergillus niger glycosidase 40 mg. The total yield of aroma constituents was 179.06×10^-4.

关 键 词：糖苷 酶水解 SDE GC—MS 

分 类 号：TS411[农业科学—烟草工业] S572