对比超声对低氧诱导因子-1α促兔缺血后肢血管新生的评价  

Evaluation of angiogenesis induced by HIF-1α in the ischemic hindlimb of rabbit using contrast-enhanced ultrasound imaging

作　　者：李明琰[1] 陈建威[1] 张新建[2] 崔凯[1] 谢佳佳[1] 陶宇[1] 吴平生[1] 

机构地区：[1]南方医科大学南方医院心内科,广州510515 [2]中国人民解放军第159中心医院麻醉科,河南驻马店463200

出　　处：《广东医学》2010年第7期804-806,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30370587)

摘　　要：目的探讨应用对比超声(contrast enhanced ultrasound,CEU)评价低氧诱导因子-1α(hypoxia-in-ducible factor-1α,HIF-1α)促兔急性缺血后肢血管新生作用的可行性。方法18只新西兰大白兔高位节扎、离断左侧股动脉及其分支建立急性后肢缺血模型,随机分成3组,生理盐水组(NS组,术后即刻肌肉注射生理盐水0.5mL)、腺病毒空载体组(Ad-blank组,腺病毒空载体2×1010PFU溶于0.5mL生理盐水中肌肉注射)、腺病毒HIF-1α组(Ad-HIF-1α组,腺病毒介导的HIF-1α2×1010PFU溶于0.5mL生理盐水中肌肉注射),每组6只。各组术后即刻及术后14d、28d对兔后肢进行CEU及彩色多普勒血流显像(colordoppler flow imaging,CDFI)检查,第28天处死动物行病理切片行CD31免疫组化染色。结果CEU与CDFI、免疫组化检查结果一致,均显示:Ad-HIF-1α组缺血后肢血管新生优于NS组和Ad-Blank组(P<0.05);CEU与CDFI检查结果相关性良好(r=0.789,P=0.000),术后28dCEU与免疫组化微血管密度所得结果相关性良好(r=0.866,P=0.000)。结论CEU检查可对兔缺血后肢血流灌注进行定量分析,反映血管新生情况,与CDFI、免疫组化检查结论一致,相关性良好。Objective To evaluate the feasibility of contrast enhanced ultrasound （CEU） in assessing the in- duced angiogenesis by hypoxia - inducible factor --1α （ HIF -1α） in hindlimb ischemia rabbit model. Methods After ligation of left femoral artery, 18 Zealand Whites Rabbits were randomly divided into 3 groups： saline group, Ad -blank group （2 ×10^10PFU） and Ad -HIF - 1 ctgroup （2×10^10PFU）. After treated with corresponding intramuscular injections, CEU and color doppler flow imaging （CDFI） were used to observe the blood perfusion and peak systolic velocity （PSV） of the hindlimb immediately after operation and on the 14th and 28th day. On the 28th day, immunohistochemistry was ear- fled out to assess the microvascular density （MVD）. Results CEU, CDFI and immunohistochemistry all revealed signifi- cantly better blood perfusion and angiogenesis in Ad - HIF - -1αtgroup than the other two groups （ P 〈 0.05 ）. Significant correlation was found between the results measured by CEU and CDFI （ r = 0. 789, P = O. 000）, as well as between CEU and immunohistochemical examinations （ r = O. 789, P = 0. 000）. Conclusion CEU has a good correlation with color doppler ultrasonography and immunohistochemistry and is a good noninvasive technique in quantifying blood perfusion.

关 键 词：对比超声 血流灌注 血管新生 

分 类 号：R36[医药卫生—病理学]