人宫颈癌HeLa细胞核内M-CSF高表达对抗肿瘤药物的影响  

Effects of Nuclear M-CSF on the Sensitivity of Anti-tumor Chemotherapeutic Drug in Human Cervical Carcinoma HeLa Cells

作　　者：陈文琦[1] 唐圣松[2] 姜浩[1] 李旎[1] 贺更生[1] 

机构地区：[1]南华大学第一附属医院,湖南衡阳421001 [2]南华大学药学与生命科学院

出　　处：《南华大学学报（医学版）》2010年第2期222-225,共4页Journal of Nanhua University(Medical Edition)

摘　　要：目的探讨人宫颈癌HeLa细胞核内M-CSF高表达对抗肿瘤药物的影响。方法体外培养转染M-CSF人宫颈癌HeLa-M细胞、HeLa细胞、转染M-CSF空载体人宫颈癌HeLa-C细胞;分别以顺铂(0.2、2.0、20μg/mL)、紫杉醇(0.2、2.0、20μg/mL)作用于HeLa-M细胞、HeLa细胞、HeLa-C细胞48 h后,MTT法检测细胞相对活性;Western blot分析胞核内M-CSF对人宫颈癌HeLa细胞Bcl-2蛋白表达的影响。结果MTT比色法测定结果显示:在顺铂和紫杉醇单药药物浓度为20、2.0μg/mL时,HeLa-M细胞相对活性明显高于HeLa细胞、HeLa-C细胞(P<0.01),而在两药单药药物浓度为0.2μg/mL时三种细胞相对活性相比无明显差别(P>0.05)。HeLa细胞与HeLa-C细胞相比无明显统计学差异(P>0.05);Western blot法检测胞核内M-CSF明显上调人宫癌HeLa细胞Bcl-2蛋白的表达(P<0.01)。结论胞核内M-CSF能增加人宫颈癌HeLa细胞的药抗性并抑制HeLa细胞凋亡。Objective To investigate effects of nuclear M - CSF on the sensitivity of anti - tumor chemotherapeutic drug in human ceiwical carcinoma HeLa cells. Methods Nuclear in M - CSF - transfected HeLa cells （ referred as to HeLa- M cell）, pCMV,cyto,myc transfected HeLa cells （referred as to HeLa- C cell） or untransfected HeLa cells （referred as to HeLa cell） were cultured in vitro. After Treating the HeLa - M cells,HeLa - C ceils and HeLa cells with cisplatin（0.2,2.0,20 μg/mL）or paelitaxel（ 0.2,2.0,20 μg/mL）for 48 h, the cells viability was determined using MTT assay. Western Blot was used to detect Bcl - 2 expression. Results The MTT assay showed that cell viability inhibition in HeLa - M cells treated by cisplatin and paclitaxel （20 μg/mL or 2.0 μg/mL） b-ere more than HeLa - C cell and HeLa cell （ P 〈 0.01 ）. However, at 0.2 μg/mL there were no defference in the three kind of cells （ P 〉 0.05 ）. Nuclear M - CSF up - regulated the expression of Bcl - 2 expression in HeLa cell（ P 〈 0.01 ）. Conclusion Nuclear M - CSF enhances chemotherapeutic drug resistance and apoptotie resistance of HeLa cells.

关 键 词：巨噬细胞集落刺激因子 人宫癌HeLa细胞 MTT法 蛋白免疫印记法 

分 类 号：R737.33[医药卫生—肿瘤]