鸡源乳杆菌的分离鉴定与培养条件优化  

Isolation,Identification of Lactobacillus from Chicken and Optimizationg of the Culture Conditions

作　　者：宁豫昌[1] 郭宏伟[1] 

机构地区：[1]郑州牧业工程高等专科学校,河南郑州450011

出　　处：《郑州牧业工程高等专科学校学报》2010年第1期12-15,共4页Journal of Zhengzhou College of Animal Husbandry Engineering

摘　　要：从健康鸡的肠道中分离、鉴定并筛选出1株耐酸和耐胆盐能力较强的乳杆菌,命名为乳杆菌L3株。采用单因素法和正交试验设计法对乳杆菌L3株的培养条件进行了优化,结果表明,乳杆菌L3株的最佳液体发酵培养基组成是4%的蔗糖、0.4%的胰蛋白胨、10%的番茄汁、0.5%β-磷酸甘油二钠、0.2%的柠檬酸铵、0.5%的乙酸钠、0.1%的吐温-80、0.1%的L-Cys盐酸盐、0.05%的MgSO4、0.02%的MnSO4,最适接种时间是16-18h,最适培养条件是温度40℃、培养时间28 h、初始pH 5.0,在此培养条件下,乳杆菌L3株的菌落总数可达8.14×10^8cfu/mL。Isolated,identified and screened out from the healthy chickens a Lactobacillus with strong tolerance of acid and bile salt,and named as Lactobacillus strains L3.Optimized the culture conditions of Lactobacillus strains L3 by Using single-factor method and orthogonal experimental design method.The results showed that best liquid fermentation medium composition of Lactobacillus strains L3 were as follows： sucrose 4%,tryptone 0.4%,tomato juice 10%,β-glycerophosphate disodium 0.5%,ammonium citrate 0.2%,sodium 0.5%,tween-80 0.1%,L-Cys hydrochloride 0.1%,MgSO4 0.05%,MnSO4 0.02%,the optimal vaccination time is 16-18h,the optimal culture conditions of temperature is 40 ℃,incubation time 28h,the initial pH5.0.In this culture conditions,the total colony of Lactobacillus strains L3 is 8.14×10^8cfu/mL.

关 键 词：乳杆菌 鸡 分离 鉴定 培养条件优化 

分 类 号：S852.612[农业科学—基础兽医学]