幽门螺旋杆菌感染对胃癌患者APE1基因表达的影响  

Effect of Hp Infection on the Expression of APE1 in Patients with Stomach Cancer

作　　者：周先勇[1] 周会新[2] 

机构地区：[1]湖南省安乡县人民医院消化内科,湖南安乡415600 [2]湖南省人民医院消化内科,湖南长沙410002

出　　处：《医学临床研究》2010年第4期602-604,共3页Journal of Clinical Research

摘　　要：【目的】分析幽门螺旋杆菌（Hp）感染与碱基切除修复基因APEI表达的关系，探讨Hp致癌的可能分子机制。【方法】标本选取自2007年12月至2008年11月本院胃镜活检病例，胃癌病例61例，同病例癌旁黏膜同时取材，胃炎病例31例。快速尿素酶法检测胃黏膜组织中Hp感染情况，免疫组织化学方法检测碱基切除修复基因APE1蛋白表达情况。【结果】胃癌组Hp感染率为52．5％（32／61）；正常黏膜组Hp感染率为54．8％（17／31），两者相比无显著性差异（P〉0．05）。胃癌组APE1蛋白的阳性表达率为78．7％（48／61），明显高于癌旁黏膜组的37．7％（23／61）（P〈0．05）及正常黏膜组的32．3％（10／31）（P〈0．05），但后两组无显著性差异（P〉O．05）。APE1蛋白在粘液癌、低分化腺癌及高中分化腺癌中的表达均无明显差异。Hp感染组和非感染组中，APE1蛋白阳性表达率在胃癌组分别为62．5％（20／32）和96．6％（28／29），感染组的表达率明显低于非感染组（Pd0．05）。【结论】胃癌和胃炎均有较高的Hp感染率。胃癌细胞的APE1蛋白表达呈上调状态，但其表达与组织分化程度无关。Hp感染可抑制APE1蛋白的表达，检测胃黏膜中APE1蛋白的表达，将有助于胃癌的预警和早期诊断。[Objective]To analyze the relationship between Hp infection and the expression of APE1 gene, and to explore the possible carcinogenesis induced by Hp infection. [Methods]Gastric biopsy specimens of 31 gastritis and 61 gastric cancer（GCa） were collected by endoscopy in our hospital from Dec. 2007 to Nov. 2008. Guick-urease method was used to detect Hp infection. Immunohistochemical method was used to detect the expression of APE1. [Results]Hp infection rates were 52.5 % （32/61）in carcinoma group and 54.8 % （17/ 31）in normal epithelium group, respectively（ P 〉0.05）. The positive rate of APE1 expression in carcinoma group being 78.7% （48/61） was significantly higher than that in surrounding epithelium group being 37.7% （23/61）（ P 〈0.05） and in normal epithelium group being 32.3% （10/31）（ P 〈0.05）, while there was no significant difference between surrounding epithelium group and normal epithelium group （ P 〉0. 05）. The positive rate of APE1 expression in carcinoma group with Hp infection being 62.5 % （20/32） was significantly lower than that without Hp infection being 96.6%（28/29） （ P 〈0.05）. [Conclusion]There are high Hp in- fection rates in both GCa and gastritis. GCa has high expression of APE1. Hp can inhibit the expression of APE1 protein. The detection of APE1 may be helpful to predict and diagnose GCa.

关 键 词：胃肿瘤/病理学 螺杆菌感染 螺杆菌 幽门 基因表达 

分 类 号：R735.2[医药卫生—肿瘤]