锌缺乏对生长期雄性大鼠海马泛素C末端水解酶L1表达的影响  

EFFECTS OF ZINC DEFICIENCY ON THE EXPRESSION OF UCH-L1 IN THE HIPPOCAMPUS OF RATS

作　　者：陆颖[1] 赵健亚 仇梁林[1] 周松林[1] 陈亚兰[2] 车鑫仁[3] 徐广飞[1] 

机构地区：[1]南通大学公共卫生学院营养与食品卫生学教研室,南通226007 [2]南通大学医学院医学信息学系 [3]杭州市疾病预防控制中心

出　　处：《现代预防医学》2010年第9期1629-1631,1635,共4页Modern Preventive Medicine

摘　　要：[目的]观察缺锌对生长期大鼠海马泛素C末端水解酶L1(UCH-L1)表达的影响。[方法]将SD大鼠随机分为正常组、缺锌配喂组和缺锌组,正常组喂饲常锌饲料(32.5mg/kg),缺锌配喂组喂饲高锌饲料(73.5mg/kg),缺锌组喂饲缺锌饲料(1.2mg/kg)。饲养4周后处死动物,取海马组织,RT-PCR法检测UCH-L1mRNA的表达水平,Westernblot法检测UCH-L1蛋白的表达水平。[结果]与正常组和缺锌配喂组比较,缺锌大鼠海马中UCH-L1mRNA和蛋白的表达水平均显著下调。[结论]缺锌大鼠海马UCH-L1的表达异常,可能是缺锌导致认知损伤的重要机制之一。[Objective] To explore the effects of zinc deficiency on the expression of UCH-L1 in hippocampus of rats. [Methods] SD rats were randomly divided into zinc normal group （ZN）, pair-fed group （PF） and zinc deficient group （ZD）. ZN group were fed with zinc- normal diet （32.5mg / kg）, PF group were fed with zinc-high diet （73.5 mg / kg）, while ZD group were fed with zinc-deficient diet （1.2 mg / kg）. The rats were sacrificed after 4 weeks and the tissue samples of hippocampus were taken immediately. The expression of UCH-L1 mRNA was detected by RT-PCR, and the expression of UCH-L1 protein was detected by Western blot. [Results ] Compared with ZN group and PF group, the expression of UCH-L1 mRNA and protein in ZD group were both down-regulated significantly. [Conclusion] The abnormal change of expressions of UCH-L1 is observed in the hippocampus of zinc deficiency rats.This might be one of the possible mechanisms of cognitive impairment induced by zinc deficiency.

关 键 词：锌缺乏 UCH-L1 海马 认知 大鼠 

分 类 号：R151.3[医药卫生—营养与食品卫生学]