颞叶癫痫大鼠不同时期海马和皮层氨基酸类递质的变化  

Variations and relationships of the amino acids in hippocampus and cortex in different phases of temporal lobe epilepsy in rat

作　　者：李敏[1] 孔德虎[1] 许奇[2] 王烈成[1] 

机构地区：[1]安徽医科大学生理学教研室,合肥230032 [2]复旦大学医学神经生物学国家重点实验室,上海200032

出　　处：《安徽医科大学学报》2010年第2期135-138,共4页Acta Universitatis Medicinalis Anhui

基　　金：教育部新世纪优秀人才支持计划(编号:NCET-06-0557);安徽省教育厅自然科学重点基金(编号:KJ2007A028);国家自然科学基金(编号:30900420);安徽省优秀青年科技基金(编号:06043090);安徽医科大学博士科研基金(编号:XJ2005006)

摘　　要：目的探讨颞叶癫痫大鼠不同时期海马和皮层内氨基酸类递质水平的变化与颞叶癫痫发生发展的关系。方法建立颞叶癫痫大鼠模型,视频监控癫痫发作行为学特征,高效液相色谱法测定脑组织中谷氨酸(Glu)、甘氨酸(Gly)和γ-氨基丁酸(GABA)含量。结果与对照组相比,在癫痫发作急性期,模型组海马Glu/GABA比值显著升高(P<0.05),Glu/Gly比值无显著变化,皮层Glu/GABA和Glu/Gly比值亦无显著变化,在癫痫发作静止期,模型组海马和皮层Glu/GABA和Glu/Gly比值均无显著变化;在癫痫发作慢性期,模型组海马和皮层Glu/GABA和Glu/Gly比值均显著性升高(P<0.05)。结论海马脑区兴奋性和抑制性氨基酸类递质水平比例的失衡可能是导致癫痫发生发展的重要机制之一。Objective To study the relationships between the variations of the amino acids at different phases of temporal lobe epilepsy in rat brain tissues and the development of temporal lobe epilepsy（TLE）.Methods The TLE model was induced.Video was used to monitor the beharviors of the model and high performance liquid chromatography was used to detect the content of glutamate （Glu）,glycine （Gly） and γ-aminobutyric acid （GABA） in brain tissues.Results Compared with the control group during the acute phase,the ratio of Glu/GABA in hippocampus increased significantly in the model group （P〈0.05）,but the ratio of Glu/Gly in hippocampus or the ratio of Glu/GABA and Glu/Gly in cortex were no significant difference.Compared with the control group during the silent phase,there was no significant difference between the ratio of Glu/GABA and Glu/Gly in hippocampus and cortex,however,the ratio of Glu/GABA and Glu/Gly in hippocampus and cortex increased significantly during the chronic phase （P〈0.05）.Conclusion The unbalance between excitatory and inhibitory neurotransmitters in hippocampus may play an important role in the TLE.

关 键 词：大鼠 癫痫 颞叶 海马 大脑皮质 色谱法 高效液相 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R977.4[医药卫生—临床医学]