丹参酮ⅡA对β淀粉样蛋白25-35片段的作用及机制研究  

The role and mechanism of TanshinoneⅡA on β-amyloid_(25-35)

作　　者：董慧敏[1] 毛善平[1] 张兆辉[1] 刘宝辉[1] 潘高峰[1] 严明敏[1] 

机构地区：[1]武汉大学人民医院神经内科,430060

出　　处：《卒中与神经疾病》2010年第2期73-75,共3页Stroke and Nervous Diseases

基　　金：国家985工程"语言科学技术与当代社会建设跨学科创新平台"资助项目(编号985yk002);国家985工程"认知与神经信息科学平台"资助项目(编号904273258)

摘　　要：目的探讨丹参酮ⅡA对β淀粉样蛋白25-35片段(Aβ25-35)诱导的褐家鼠肾上腺嗜铬瘤细胞PC12细胞系抑制中的作用及机制。方法MTT检测丹参酮ⅡA(2μmol/L)对Aβ25-35(20mmol/L)处理过的细胞活性,应用公式(1-实验组平均OD值/对照组OD值)×100%计算细胞抑制率。RT-PCR、westernblot检测细胞内GSK3β及pi GSK3β的表达量。结果丹参酮ⅡA有效的拮抗了Aβ25-35引起的细胞抑制及GSK3β的高表达和低磷酸化。结论Aβ25-35可以引起PC12细胞抑制,而丹参酮可以拮抗Aβ25-35的作用,并且可能是通过GSK3β通路而发挥作用。Objective To explore the role and the probabily mechanism of tanshineⅡA on β-amyloid25–35.Methods Application of MTT was used to detect the activity of PC12 cells.The formula（1-treatment group OD/control group OD） ＊100% was used to Calculate PC12 cells growth inhibition rate.Reverse transcription-PCR（RT-PCR） and western blot were used to observe the expression of GSK3β and piGSK3β.Results TanshineⅡA could effectively ease the cell inhibition induced by the Aβ25-35,the high expression of GSK3β and the low expression of piGSK3β.Conclusions Aβ25-35 could induce inhibition in PC12 cells and tanshineⅡA could ease the inhibition induced by the Aβ25-35 through GSK3 pathway.

关 键 词：β淀粉样蛋白25-35片段 阿尔茨海默病 糖原合酶激酶3Β 丹参酮ⅡA 

分 类 号：R742[医药卫生—神经病学与精神病学]