海带多糖对多次小剂量电离辐射致大鼠睾丸组织损伤的影响  

Study of the intervention effect of Laminaria japonica polysaccharides on testis tissue in male rats exposed by repeated low-dose ionizing radiation

作　　者：刘军[1] 罗琼[1] 崔晓燕[1] 杨明亮[1] 阎俊[1] 

机构地区：[1]武汉大学公共卫生学院,430071

出　　处：《中华放射医学与防护杂志》2010年第2期173-176,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：达能膳食营养与宣教基金（DIC2007-06）

摘　　要：目的探讨海带多糖（LJP）对多次小剂量电离辐射导致的大鼠睾丸组织氧化损伤的影响。方法将Wistar雄性大鼠随机分为正常对照组、辐射模型组和LJP干预组；LJP干预组给予LJP，其余各组以等量生理盐水灌胃；除对照组外，各组在适应性饲养7d后进行^60Co γ射线局部照射，1次／d，4周20次，总剂量2．3Gy／只；分别于停止照射后1、7和14d处死大鼠，分离大鼠睾丸、附睾等组织；采用可见光分光光度计比色法检测睾丸丙二醛（MDA）、谷胱甘肽（GSH）含量及超氧化物歧化酶（SOD）、乳酸脱氢酶（LDH）和谷胱甘肽过氧化物酶（GSH—PX）活性；光镜观察睾丸组织形态；统计其精子数量及存活率。结果LJP各组与对应模型各组比较，睾丸中MDA含量减少（t=3．66～5．03，P〈0．01），GSH含量增加，SOD、LDH和GSH—PX活性增强（t=2．77～25．52，P〈0．05）；睾丸组织损伤减轻；精子数量及存活率提高（t=3．11～23．08，P〈0．01）；停止辐射14d后的各项指标更接近对照组。结论LJP能促进多次小剂量局部电离辐射导致的睾丸组织氧化损伤恢复，对睾丸组织的修复及其生精功能亦有促进作用。Objective To investigate the effect of the Laminaria japonica polysaccharides（LJP） intervention on the male rats＇ testicular tissue oxidative damage which was caused by repeated low-dose local ionizing radiation. Methods Male Wistar rats were randomly divided into control group, model group and LJP intervention group. The rats in the LJP intervention group was given with LJP. The rats in each group were exposed to 60Co ~/-ray local irradiation 7 d after adaptability breeding, once per day for 20 times in 4 weeks. Each rat was irradiated with the total dose of 2.3 Gy. The rats were killed at 1, 7 and 14 d post-irradiation, respectively. The testis and the epididymis were taken out. The contents of MDA and GSH, and the activities of SOD, LDH and GSH-Px were measured using spectrophotometer. The amorphous of testicular tissue was observed and the sperm count and viability were analyzed. Results As compared with those in the model group, the content of MDA decreased in testicular tissue in the LIP group （t = 3.66-5.03, P 〈 0. 01 ） , while the GSH content increased. The activities of SOD, LDH and GSH-Px （ t = 2.77-25.52, P 〈 0. 05 ） and the sperm count and viability increased （ t = 3. 11-23.08, P 〈 0.01 ）. Each index was more close to that in the control group 14 d post-irradiation. Conclusions LJP can promote the recovery of testicular tissue oxidative damage caused by chronic local ionizing radiation. It has a role in promoting the spermatogenic function of male rats.

关 键 词：海带多糖 电离辐射 氧化损伤 大鼠 睾丸 

分 类 号：R285.5[医药卫生—中药学]