所谓肺硬化性血管瘤的克隆性的研究  

Study on clonality and loss of heterozygosity in pulmonary sclerosing hemangioma

作　　者：张丽[1] 巩丽[1] 李艳红 任凯夕[1] 姚丽[1] 兰淼[1] 朱少君[1] 韩秀娟[1] 王军[1] 张伟[1] 

机构地区：[1]第四军医大学唐都医院病理科,陕西西安710038

出　　处：《现代肿瘤医学》2010年第5期852-854,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:30800417)

摘　　要：目的:探讨所谓肺硬化性血管瘤(PSH)组织内及整个病变的克隆性组成及意义。方法:选PSH患者手术切除标本29例,全部为女性。石蜡切片HE染色,应用激光捕获显微切割技术获取PSH组织中多角形细胞和表面立方细胞,分别提取基因组DNA,用甲基化敏感的限制性内切酶HhaⅠ消化,巢式聚合酶链反应扩增X染色体连锁的雄激素受体(AR)基因。AR基因产物经变性聚丙烯酰胺凝胶电泳、银染显示其长度多态性。结果:光镜下可见29例PSH均表现其典型的组织病理学特征:主要由实性、乳头状和血窦所构成,其中实性区细胞大小基本一致。高倍镜下可见实性区细胞呈圆形或多角形,胞质呈嗜酸性或略呈透明状,核分裂罕见;乳头状结构表面均被覆单层立方上皮。克隆性检测结果表明有23例显示AR基因位点具有多态性。经HhaI酶切后,所有具有多态性的标本2个条带中有1个条带的密度明显减弱或消失,而另一个条带保留,证明其均为肿瘤性增生。结论:PSH组织中的多角形细胞和表面立方细胞具有相同的单克隆增生模式,提示二者可能均为肿瘤的实质细胞。Objective：To investigate the elonality composition and significance of the so - called pulmonary scle- rosing hemangioma （PSH）. Methods：Polygonal and surface cuboidal cells were microdissected from PSH lesion in 29 female patients. Genomie DNA were extracted, pretreated by methylation - sensitive restrictive endonuclease Hpa I, and then AR gene was amplified via nested polymerase chain reaction （PCR） based on X - chromosome inactivation mosai. Length polymorphisms of AR gene product were shown by denatured polyacrylamide gel eleetrophoresis and sil- ver staining. Results：Microscopically, typical histopathological features were observed in all the 29 cases, composed by solid, papillary and blood sinus. The cells in solid region were round or polygonal, cytoplasm was acidophilia or slightly transparent. Karyokinesis was hardly to observe, the surface of papillary region were covered with simple cuboidal epi- thelium. Cells in solid region were in similar size. Clonality assay showed AR gene polymorphism in 23 cases. Follow- ing digestion by HhaI, density of one band in all samples which had polymorphism significantly decreased or disap- peared,but remained in the other band, which confirmed they were neoplastic proliferation. Conclusion： Polygonal cells and surface cuboidal ceils of PSH lesion have the same monoclonal proliferative pattern. This suggests they are neoplastic parenchymal cells.

关 键 词：单克隆 硬化性血管瘤 肺肿瘤 雄激素受体 

分 类 号：R730.231[医药卫生—肿瘤]