高温联合顺铂及多西他赛对肺腺癌A549细胞体外作用的研究  

The effect of hyperthermia combined with cisplatin and docetaxel on human lung cancer cell line A549 in vitro

作　　者：刘睿[1] 郭姗[1] 闵婕[1] 康艳霞[1] 张贺龙[1] 

机构地区：[1]第四军医大学唐都医院肿瘤科,陕西西安710038

出　　处：《现代肿瘤医学》2010年第5期857-861,共5页Journal of Modern Oncology

摘　　要：目的:研究高温联合顺铂(DDP)及多西他赛(TXT)对肺腺癌细胞株A549的体外作用。方法:不同处理因素作用于A549细胞后,应用四氮唑盐比色法(MTT法)测定细胞增殖情况,根据Veleriote法判断高温联合化疗药的作用效果;通过两药相互作用指数判断药物联合作用效果;流式细胞仪检测不同处理后细胞凋亡情况;光学显微镜及荧光显微镜观察细胞形态学变化。结果:高温和化疗药单独作用均对A549有生长抑制作用(P<0.05);化疗药有剂量依赖关系;高温有温度依赖关系;高温与药物联合对细胞生长抑制作用强于单独高温组或单独化疗组(P<0.01);DDP与高温联合为协同作用;TXT与高温联合为次加作用;DDP和TXT联合作用对细胞生长抑制为协同作用;42℃、DDP2μg/ml和TXT1μg/ml三者联合凋亡率明显高于其他处理组(P<0.01);普通光镜和荧光显微镜下42℃、DDP2μg/ml和TXT1μg/ml三者联合凋亡细胞数较对照组和42℃组明显增多,细胞形态学变化明显。结论:DDP、TXT、高温三者体外联合作用对A549有明显的生长抑制和诱导凋亡作用。Objective：To study the synergistic effect of combined use of cisplatin, docetaxel and hyperthermia on human lung adenocarcinoma A549 cell in vitro. Method ： A549 cell was treated with different drugs , temperatures. Cell viability and growth were measured by means of MTr assay. The effect of combined use of cisplatin, docetaxel and hyperthermia were examined by means of morphologic observation and flow cytometry （FCM）. Results： The signifi- cantly proliferation inhibition effect were observed when using either hyperthermia or drugs alone（ P 〈 0.05 ） ,with a temperature or dose - dependent manner. Hyperthermia enhanced the proliferation inhibition rates compare with drug alone（P 〈0.01 ）. Significant synergistic effect was observed when cisplatin combined with docetaxel. The apoptosis ratio of combined use of cisplatin 2tLg/ml,docetaxel 1 ~g/ml at 42~C was higher than other groups. The results were coincident with morphologic observation. Conclusion： Synergistic antitumor effects of combined use of cisplatin, do- cetaxel and hyperthermia on A549 were prior to other groups , including significantly proliferation inhibition and apop- tosis induction.

关 键 词：顺铂 多西他赛 高温 肺癌 

分 类 号：R730.5[医药卫生—肿瘤]