血小板反应蛋白-1的表达与骨肉瘤新生血管生成的研究  

The research to expression of thrombospodin-1 and angiogenesis of Osteosarcoma

作　　者：宋玉鑫[1] 刘守正[2] 张辉[1] 胡红霞[1] 段大鹏[3] 王建民[4] 

机构地区：[1]甘肃省人民医院,甘肃兰州730000 [2]沭阳县人民医院,江苏沭阳223600 [3]陕西省人民医院,陕西西安710068 [4]兰州大学第一医院,甘肃兰州730000

出　　处：《现代肿瘤医学》2010年第5期983-986,共4页Journal of Modern Oncology

摘　　要：目的:检测血小板反应蛋白-1(thrombospodin-1,TSP-1)在骨肉瘤中的表达,探讨其与骨肉瘤组织血管生成、侵袭性的关系。方法:收集2003年1月-2007年2月手术切除的54例骨肉瘤标本,应用免疫组化法,检测骨肉瘤组织中TSP-1的表达,CD34单克隆抗体标记血管内皮细胞计数骨肉瘤微血管密度(mi-crovessel density,MVD)。结果:骨肉瘤TSP-1的表达程度与MVD呈负相关,TSP-1高表达组的MVD明显低于TSP-1低表达组;骨肉瘤TSP-1表达与骨肉瘤临床病理因素无相关性,但与肺转移显著负相关。结论:TSP-1在骨肉瘤发生发展中起重要作用,并影响血管形成及肿瘤侵袭性,有望成为治疗骨肉瘤的新靶点。Objeclive：To explore the expression of thrombospodin - 1 （TSP - 1 ） in osteosarcoma and investigate its relationship with tumor angiogenesis. Methods：The expression of TSP- 1 was evaluated by immunohistochemical staining on tumor specimens from 54 patients with osteosarcoma. The microvessel density（MVD） was assessed by im- munostaining with CD34 in the most eovascularized area （hot spots）. Results： In osteosarcoma,the mean microves- sel count of TSP - 1 high - expression tumors was significantly lower than that of TSP - 1 low - expression tumors. The close correlation was found between TSP - 1 expression and microvessel density. TSP - 1 expression and microvessel density was negatively correlated with lung metastasis in osteosarcoma. In contrast,no association was found between TSP - 1 expression and gender, age, size and location of tumor, osteosarcoma classification, as well as micmvessel density. Conclusion： TSP - 1 may play an important role in the development of osteosarcoma, and TSP - 1 regulates invasiveness and angiogenesis. Thus, TSP - 1 might be a new therapy target in osteosarcoma.

关 键 词：骨肉瘤 血小板反应蛋白-1 微血管密度 肺转移 

分 类 号：R738.1[医药卫生—肿瘤]