MTHFR基因677C/T多态性对精子密度的影响  

Effect of Polymorphism of 677C/T in MTHFR Gene on Sperm Count

作　　者：阿周存[1] 张炜[2] 

机构地区：[1]大理学院生命科学与化学学院,大理671000 [2]四川大学华西医院医学遗传室,成都610041

出　　处：《生殖与避孕》2010年第3期183-186,共4页Reproduction and Contraception

基　　金：国家自然科学基金项目;项目号:30770809

摘　　要：目的:探讨MTHFR基因677C/T的多态性对人类精子密度的影响。方法:应用PCR和限制性片段长度多态(RFLP)分析的方法在280例精子密度正常的男性个体中,对MTHFR基因677C/T位点的基因型分布进行调查,并评估不同基因型个体的平均精子密度。结果:在280例男性中,基因型CC、CT和TT的个体数分别为145、104和31,其平均精子密度分别为70.8±33.9×106/ml、67.6±31.2×106/ml和60.2±24.5×106/ml;基因型CC的精子密度显著高于TT型(P<0.05)。结论:MTHFR基因677C/T多态性可能对精子密度产生影响,降低精子密度。Objective：To explore the effect of polymorphism of 677C/T in MTHFR gene on sperm count in human.Methods：The genotype of 677C/T locus in MTHFR gene was investigated in 280 men with normal sperm count using polymerase chain reaction（PCR） and fragment length polymorphism analysis（RFLP）,and average sperm count of each genotype was estimated.Results：In 280 men,the individual number of genotype CC,CT and TT was 145,104 and 31 and the average sperm count of genotype CC,CT and TT was 70.8 ± 33.9 × 10^6/ml,67.6 ± 31.2 × 10^6/ml and 60.2±24.5 ×10^6/ml,respectively.The sperm count of genotype CC was significantly higher than that of genotype TT（P0.05）.Conclusion：The polymorphism of 677C/T in MTHFR may decrease sperm count.

关 键 词：MTHFR基因 多态性 精子密度 

分 类 号：R394[医药卫生—医学遗传学]