DNA修复基因XPC和XPG单核苷酸多态性与肝细胞癌易感性的关系  

Association of XPC and XPG polymorphisms with the risk of hepatocellular carcinoma

作　　者：李龙嫚[1] 曾小云[1] 纪龙[1] 范雪娇[1] 李永强[2] 胡晓桦[2] 仇小强[3] 余红平[1] 

机构地区：[1]广西医科大学公共卫生学院流行病学与卫生统计学教研室,南宁530021 [2]广西医科大学附属肿瘤医院化疗科 [3]桂林医学院

出　　处：《中华肝脏病杂志》2010年第4期271-275,共5页Chinese Journal of Hepatology

基　　金：国家自然科学基金（30660162）;广西科学研究与技术开发计划（桂科攻0719006-2-13）;广西研究生教育创新计划（2008105981004M189）;广西大型仪器协作网测试补助（668-2008-081）

摘　　要：目的探讨广西地区DNA修复基因XPC（Ata499Val、Lys939Gln）和XPG（His1104Asp）单核苷酸多态性与肝细胞癌（HCC）易感性的关系。方法采用以医院为基础的病例对照研究。经组织病理学确诊的HCC患者500例，相同地区，年龄、性别和民族频数匹配的非肿瘤患者507例。采用TaqMan MGB实时荧光定量PCR检测XPC和XPG基因多态性，比较不同基因型与HCC患病风险的关系。分别用t检验和Х^2检验对计量资料和计数资料进行统计分析；采用非条件的Logistic回归计算比值比（OR）及其95％可信区间（CI）。结果与XPC基因Ala499Val位点CC基因型相比，CT或者TT基因型与HCC患病风险无相关性（校正OR=1．34，95％CI：0．85～2．12；校正OR=1．30，95％强0．68～2．51）；与Lys939Gln位点AA基因型相比，AC或者CC基因型与HCC患病风险无相关眭（校正OR=1．20，95％ CI：0．78～1．85；校正OR=1．81，95％CI：0．88～3．73）。与XPG基因His1104Asp位点CC基因型相比，CG或者GG基因型与HCC患病风险无相关性（校正OR=0．85，95％C／：0．56～1．27；校正OR=1．12，95％吖：0．67～1．87）。分层分析结果显示，与携带XPC基因Lys939Gln位点AA基因型女性相比，携带AC＋CC基因型的女性患HCC的风险增加2．17倍（95％CI：1.01～4．64）。结论XPC基因Ala499Val和Lys939Gln位点以及XPG基因His 1104Asp位点SNP的单独效应可能与HCC易感性无相关性，但是XPC基因Lys939Gln位点C等位基因可协同增加女性患HCC的风险。Objective To investigate whether the polymorphism of DNA repair genes XPC （Ala499Val and Lys939Gln） and XPG （His1104Asp） is associated with the susceptibility to hepatocellular carcinoma （HCC）. Methods A hospital-based case-control study was conducted in 500 cases with HCC and 507 controls. Genotypes of XPC and XPG were determined by real-time polymerase chain reaction with the TaqMan MGB probe. Results Compared to the CC genotype, the CT genotype and the Tr genotype of XPCAla499Val were not associated with the susceptibility to HCC （adjusted OR = 1.34, 95% CI： 0.85-2.12; adjusted OR = 1.30, 95% CI： 0.68-2.51, respectively）. Compared to the AA genotype, the AC genotype and the CC genotype of Lys939Gln were not associated with the susceptibility to HCC （adjusted OR = 1.20, 95% CI： 0.78-1.85; adjusted OR = 1.81, 95% CI： 0.88-3.73, respectively）. Compared to the CC genotype, the CG genotype and the GG genotype ofXPG His1104Asp were not associated with the susceptibility to HCC （adjusted OR = 0.85, 95% CI： 0.56-1.27; adjusted OR = 1.12, 95% CI： 0.67-1.87, respectively） However, the stratified analysis revealed that the females with the AC＋CC genotype of XPC Lys939Gln had increased risk of HCC compared to those with AA genotype （OR = 2.17, 95% CI： 1.01-4.64）. Conclusion Our results suggest that XPC and XPG polymorphisms do not independently affect on the susceptibility to HCC, but the joint effect of C allele of XPC Lys939Gln and female may modify the risk of HCC.

关 键 词：癌 肝细胞 多态性 单核苷酸 XPC基因 XPG基因 

分 类 号：R735.7[医药卫生—肿瘤]