5氮杂胞苷对长春新碱及环磷酰胺诱导神经母细胞瘤细胞凋亡的调节作用  

Regulating action of 5AZA to the chemotherapy sensitivity of neuroblastoma

作　　者：张家魁[1] 王维林[1] 张锦华[1] 李晓霞[1] 

机构地区：[1]中国医科大学附属第四医院胃肠外科,辽宁沈阳110032

出　　处：《中华肿瘤防治杂志》2010年第1期17-19,36,共4页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究5氮杂胞苷(5AZA)使神经母细胞瘤(NB)细胞重新表达caspase-8蛋白的作用,以及重新表达caspase-8的NB细胞对传统化疗药长春新碱和环磷酰胺敏感性的变化。方法:细胞免疫组化染色方法检测5AZA作用前后NB细胞caspase-8蛋白的表达情况。MTT细胞毒实验方法检测caspase-8蛋白表达前后NB细胞经长春新碱及环磷酰胺作用后细胞凋亡率的改变。结果:免疫组化表明,经过5AZA作用后,NB细胞重新表达caspase-8蛋白。MTT细胞毒实验结果表明,经5AZA作用后重新表达caspase-8蛋白的NB细胞经长春新碱及环磷酰胺的作用后,凋亡率较未表达caspase-8蛋白的NB细胞明显上升,P<0.05。结论:5AZA可以使NB细胞的caspase-8蛋白恢复表达,而恢复表达caspase-8的NB细胞可以恢复对化疗药长春新碱及环磷酰胺的敏感性。OBJECTIVE：To investigate the effect of 5AZA which can make NB cells resume caspase-8 expression,and study whether the NB cells which have expressed caspase-8 can resume the chemotherapeutic sensitivity. METHODS：The expressions of caspase-8 protein before and after treatment of 5AZA were detected by cell immunochemistry method. The MTT method was used to analyse that whether the NB cells which expressed caspase-8 could resume the chemotherapeutic sensitivity. RESULTS：Cell immunochemistry showed the expression of caspase-8 protein was undetectable in NB cells and it was positive after treatment by 5AZA. The MTT analysis showed the expression of caspase-8 protein could made the SY5Y resume the sensitivity to CTX and VCR significantly（P〈0.05）. CONCLUSIONS：5AZA can regulate the expression of caspase-8 protein of NB cells. Expression of caspase-8 protein can make the NB cells resume the sensitivity to CTX and VCR.

关 键 词：神经母细胞瘤 阿扎胞苷/药理学 细胞凋亡/药理作用 

分 类 号：R739.4[医药卫生—肿瘤]