下咽癌FADU细胞系中CD133阳性细胞的侵袭能力  

Invasion of the CD133-positive cells in the FADU cell line

作　　者：梁辉[1,2] 潘新良[1] 马超[3] 王启荣[2] 韩飞[2] 崔朝阳[2] 

机构地区：[1]山东大学齐鲁医院耳鼻咽喉科,济南250012 [2]山东大学附属千佛山医院耳鼻咽喉科,济南250014 [3]山东大学卫生部耳鼻喉科学重点实验室,济南250012

出　　处：《山东大学耳鼻喉眼学报》2010年第2期5-7,共3页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的研究下咽癌人咽鳞癌细胞(FADU)细胞系中CD133阳性细胞侵袭能力。方法用免疫磁珠分选技术,分选CD133阳性细胞及阴性细胞,Transwell小室方法研究阳性、阴性二个细胞亚群的体外侵袭能力。结果阳性、阴性细胞在Transwell小室侵袭实验中穿过人工基底膜的数量分别为54.0±2.3,17.0±1.1,其差异有统计学意义。结论下咽癌FADU细胞系中,CD133阳性细胞具有更强的侵袭能力。Objective To investigate the invasion of the CD133-positive cells in the FADU cell line.Methods In our study,immunomagnetic beads were applied to purify CD133-positive cells in the FADU cell line.Invasions of CD133-positive and CD133-negative cells were measured by the Transwell experiment.Results The number of cells infiltrating the Transwell membrane in the CD133-positive group（54±2.3）was significantly higher than that in the CD133-negative group（17±1.1）.Conclusion CD133-positive cells in the FADU cell line show higher invasive ability.

关 键 词：下咽癌 肿瘤干细胞 侵袭 CD133 

分 类 号：R739.63[医药卫生—肿瘤]