CCL22-CCR4轴在胃癌腹膜乳斑转移的机制研究  

MDC/CCL22-CCR4 axis in milky spots in peritoneal carcinomatosis of gastric cancer

作　　者：黄罡[1] 胡祥[1] 曹亮[1] 罗红[2] 

机构地区：[1]大连医科大学附属第一医院普外科,辽宁省116011 [2]大连医科大学免疫微生物检验教研室

出　　处：《中华普通外科杂志》2010年第4期322-325,共4页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目（30873036）

摘　　要：目的探讨巨噬细胞衍生因子MDC／CCL22-CCR4反应轴在胃癌腹膜乳斑转移中的作用。方法用RT-PCR和Westernblot方法检测CCR4在5种胃癌细胞系中的表达；MTT法测定不同浓度CCL22对胃癌细胞株BGC-823增殖率的变化；Transwell小室体外侵袭实验检测CCL22对胃癌细胞株BGC-823趋化活性的影响；免疫组化检测标本中CCR4表达；单变量分析CCR4表达与胃癌临床病理参数的关系。结果CCR4mRNA和CCR4蛋白在5种胃癌细胞系中均有表达。CCL22可以促进BGC-823细胞的增殖和趋化。CCR4在胃癌中有表达，CCR4表达水平与胃癌分化程度相关（P〈0．05），而与患者的年龄、肿瘤大小、位置、分期以及淋巴结转移等均无关（P〉0．05）。结论CCL22-CCR4反应轴可能在胃癌腹膜乳斑转移中起作用。CCR4可能成为预防和治疗胃癌腹膜转移的潜在靶点。Objective To study the role of MDC/CCL22-CCR4 axis in milky spots in peritoneal carcinomatosis of gastric cancer. Methods We examined the expression of CCR4 in 5 gastric cancer cell lines by RT-PCR and Westernblot. Cell growth rate of BGC-823 cell lines before and after incubated with CCL22 was detemined by MTF assay. The effects of CCL22 on invasion of BGC-823 cells into Matrigel were measured using Transwell test. Immunohistochemistry was conducted to detect the expression of CCR4. Results CCR4 mRNA and protein expression were detected in all 5 human gastric cancer cell lines ; CCL22 had a direct promotive effect on BGC-823 cell proliferation and invasion, There was CCR4 expression in Stomach neoplasms. The expression of CCR4 in primary tumor was correlated with tumor pathological differentiation （ P 〈 0. 05 ）, but not with age of patients, gender, location of tumor, tumor size, tumor stage, lymph node metastasis （ P 〉 0. 05 ）. Conclusions The MDC/CCL22-CCR4 axis may be one of the mechanism of peritoneal metastasis in milky spot in gastric cancer.

关 键 词：胃肿瘤 肿瘤转移 趋化因子 巨噬细胞 乳斑 

分 类 号：R735.2[医药卫生—肿瘤]