支气管败血波氏杆菌、多杀性巴氏杆菌和副猪嗜血杆菌同时检测的PCR方法的建立  

Establishment of a PCR assay for detecting Bordetella bronchiseptica , Pasteurella multocida and Haemophilus parasuis

作　　者：邸海波[1] 楚常欢[1] 宋苍浩[1] 张玥莹[1] 杨平东[1] 黄伟[1] 

机构地区：[1]西南大学荣昌校区,重庆荣昌402460

出　　处：《畜禽业》2010年第5期57-59,共3页Livestock and Poultry Industry

摘　　要：在已经建立的支气管败血波氏杆菌(Bb)、多杀性巴氏杆菌(Pm)、副猪嗜血杆菌(HPS)的单项PCR诊断方法的基础上,通过对扩增条件的优化,成功建立在一个循环条件下同时检测3种细菌的PCR实验室诊断方法。利用一次PCR即可同时扩增出Bb的237bp、Pm的457bp,和HPS的821bp的特异性片段。该方法可检测到1.34×103cfu1/mLBb,1.6×102cfu/mLHPS,2.6×102cfu/mLPm。该方法的敏感性与已报道的各单项PCR方法的敏感性一致。该方法的建立对临床上进行这3种疾病的鉴别诊断和混合感染的检测都具有重要意义。A PCR detecting Bb, Pm and HPS was developed based on published single PCRs for these pathogens. The PCR reagents, annealing temperature and the PCR procedure were optimized. A 237 bp fragment of Bb, a 457 bp of Pm and a 821 bp of HPS can be simultaneously amplified in one condition .The PCR can detect minimum 1.34×103cful/mL cells of Bb,2.6×102cfu/mL cells of Pm, 1.6×102cfu/mL cells of HPS. The sensitivity of the PCR is quite comparable with their reported uniplex PCRs.The assay is a potential tool for differentiation of the 3 pathogens and diagnosis of mixed infection by these bacteria.

关 键 词：BB HPS Pm PCR 

分 类 号：S858.28[农业科学—临床兽医学]