人骨肉瘤OS-732单克隆抗体的制备及纯化  

Preparation and purification of murine monoclonal antibody against human osteosarcoma OS-732

作　　者：贾艳辉[1] 陈继营[1] 崔雪梅[1] 眭翔[1] 郭全义[1] 张罕[1] 许文静[1] 张莉[1] 袁玫[1] 卢世璧[1] 

机构地区：[1]解放军总医院骨科研究所,北京100853

出　　处：《军医进修学院学报》2010年第5期462-464,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家"863"计划项目(2007AA021806)~~

摘　　要：目的制备、纯化抗人骨肉瘤OS-732单克隆抗体。方法通过杂交瘤细胞腹腔注射BALB/c小鼠制备腹水,ProteinA-Sepharose CL-4B亲和层析法纯化单抗,SDS-PAGE、免疫组化及ELISA检测该单抗的性质。结果SDS-PAGE鉴定单抗纯度达到93%,ELISA检测抗体免疫活性效价为1×10-7。结论制备、纯化了具有较高纯度及生物活性的的抗骨肉瘤OS-732单克隆抗体,为下一步骨肉瘤靶向治疗打下了基础。Objective To prepare and purify the murine antibody against human osteosarcoma OS-732.Methods Human osteosarcoma OS-732 and hybridoma cells were injected into the abdominal cavity of BALB/c mice to produce monoclonal antibody against human osteosarcoma.The antibody was purified by protein A-sepharose CL-4B affinity chromatography and its properties were evaluated by SDS-PAGE,immunohistochemistry and ELISA.Results SDS-PAGE showed that the purity of monoclonal antibody was about 93%.ELISA revealed that the immunologic activity of monoclonal antibody was 1×10-7.Conclusion Monoclonal antibodies with a high purity and immunological activity can be employed in further study of targeted therapy for human osteosarcoma.

关 键 词：骨肉瘤 杂交瘤 抗体 单克隆 亲和层析 

分 类 号：R738.1[医药卫生—肿瘤]