Seldi-Tof-MS技术检测大鼠抗Thy-1模型的血清差异多肽  

Detection of differential peptides in serum of rat anti-Thy-1 model by Seldi-Tof-MS

作　　者：吕杨[1] 谢院生[1] 张术佳[1] 陈香美[1] 

机构地区：[1]解放军总医院肾病科全军肾脏病研究所肾脏病重点实验室,北京100853

出　　处：《军医进修学院学报》2010年第5期472-474,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金重点项目资助(30630033)~~

摘　　要：目的利用生物大分子表面加强的激光解析/电离时间飞行质谱技术(Seldi-Tof-MS)检测大鼠抗Thy-1模型在系膜增殖期血清中的差异多肽,寻找与系膜增殖相关的生物标志物。方法12只200g雄性Wistar大鼠,分为对照组和模型组,对照组尾静脉注射0.9%氯化钠注射液,模型组尾静脉注射抗Thy-1抗体,7d后处死。过碘酸-希夫(PAS)染色检测病理变化,Seldi-Tof-MS检测血清中差异多肽。结果模型组在第7天出现明显的系膜细胞增殖和基质积聚,与对照组相比,模型组有两个多态峰发生变化(P<0.01),其中质荷比为2746ku的多肽在模型组高表达,7399ku在模型组低表达。结论利用Seldi-Tof-MS在抗Thy-1模型血清中成功发现了两个差异多肽,他们可能是系膜增殖相关生物标志物。Objective To detect the differential peptides in serum of rat anti-Thy1 nephritis model during their proliferation in mesentery by Seldi-Tof-MS in order to find mesangial cell proliferation-related biomarkers.Methods Twelve male Wistar rats weighing 200g were divided into control group and model group.Rats in control group were injected with physiological saline and rats in model group were injected with anti-Thy1 antibody through tail vein.Animals were killed 7 days later.Periodic acid-Schiff（PAS） staining was used to detect pathologic changes in the two groups and Seldi-Tof-MS was used to detect differential peptides in serum of rat anti-Thy1 nephritis model.Results Significant proliferation of mesangial cells and extracellular matrix accumulation were observed in model group 7 days later.Two differential peptides were found in model group with a high expression level of M/Z 2 746ku peptide and a low expression level of M/Z 7 399ku（P0.01）.Conclusion Two differential peptides can be found in serum of anti-Thy1 nephritis model using Seldi-Tof-MS,which may be the mesangial cell proliferation-related biomarkers.

关 键 词：抗原 Thy-1 肾炎 蛋白质组学 表面增强激光解析离子化蛋白质芯片 

分 类 号：R730.4[医药卫生—肿瘤]