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机构地区:[1]运城学院生命科学系,山西运城044000 [2]西北大学生命科学学院,西部资源生物与现代生物技术省部共建教育部重点实验室,陕西西安710069 [3]运城学院生化实验中心,山西运城044000
出 处:《山地农业生物学报》2010年第2期95-99,共5页Journal of Mountain Agriculture and Biology
摘 要:利用SDS-PAGE技术,分析了晋南麦区近10年来主要推广的38份小麦骨干品种的高分子量麦谷蛋白亚基(HMW-GS)组成及其与GMP含量、膨胀势、降落值及直链淀粉含量的关系。结果表明,Glu-1位点亚基对晋南小麦骨干品种面包品质性状的作用主要表现在GMP含量及GMP/pro上,对淀粉品质性状有显著作用的只有14+15(正相关)和5+10(正相关)两个亚基。5+10亚基不仅与GMP含量及GMP/pro呈极显著正相关,且与膨胀势呈极显著正相关;2*、20、4+12亚基与所列品质性状指标相关均不显著。The high molecular weight (HMW) glutenin subunit compositions of 38 wheat germplasm accessions, which had been popularized in South Shanxi during past decade, were determined after grain proteins were fractionated by polyacrylamide gel eleetrophoresis in the and the relationships with their bread- making qualities were suhs indicated that the effect of the subunits on bread - baking presence of sodium dodecyl sulphate (SDS) , also investigated in the current work. The requalities of wheats in South Shanxi was mainly manifested in GMP content and the proportion of GMP in the whole protein. Subunits 14 + 15 ( positive correlation) and 5 + 10 ( positive correlation) had significant effect on starch qualities. Subunits 5 + 10 were significantly correlated to GMP content and the proportion of GMP in the whole protein, as well as to swelling power. No significant correlation was obtained between three subunits (2^* ,20 and 4 + 12) and these properties.
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