黄连吴茱萸药对水提物对大鼠结肠癌癌前病变的作用  

Effects of Coptis chinensis and Evodia rutaecarpa water extract on DMH-induced precancerous lesion of colon

作　　者：董立[1,2] 石海莲[1,2] 季光[3] 吴大正[1,2] 

机构地区：[1]上海中医药大学中药研究所,上海201203 [2]上海市复方中药重点实验室,上海201203 [3]上海中医药大学龙华医院脾胃病研究所,上海200032

出　　处：《中国中药杂志》2010年第9期1185-1188,共4页China Journal of Chinese Materia Medica

基　　金：上海市复方中药重点实验室开放课题基金项目(09DZ2270900);上海市自然科学基金项目(09ZR1431800);上海市教委高校高水平项目建设资助项目(2008GSP19)

摘　　要：目的:研究黄连吴茱萸药对对DMH诱导的大鼠结肠癌癌前病变以及结肠上皮增殖和凋亡的作用。方法:采用DMH制备大鼠结肠癌模型;应用形态学的方法测定经过细微分离获得的完整肠腺内细胞的增殖和凋亡变化;应用甲基绿染色和光学显微镜计数中、降结肠异变肠腺病灶数目。结果:黄连吴茱萸药对能够明显抑制模型组动物ACF的形成。模型组动物中、降结肠肠腺内细胞增殖显著增加,而黄连吴茱萸药对能够明显抑制中、降结肠肠腺内细胞的增殖;模型组动物降结肠肠腺内细胞凋亡明显增加,而中结肠肠腺内细胞凋亡较正常相比无明显差异,而黄连吴茱萸药对能够显著促进中、降结肠肠腺内细胞的凋亡。结论:黄连吴茱萸药对能够显著抑制结肠癌癌前病变ACF的形成,提示其对结肠癌可能具有抑制和临床治疗作用,其作用部分通过抑制细胞增殖和促进细胞凋亡实现。Objective： To investigate the effects of Coptis chinensis and Evodia rutaecarpa water extract on precancerous lesion of colon induced by DMH and proliferation and apoptosis changes of colon mucosa crypts. Method： Precancerous lesion of colon was induced by DMH. The changes of proliferation and apoptosis of colon mucosa crypts were detected by morphological analysis. The numbers of aberrant crypt foci （ACF） were measured by feulgen staining. Result： C. chinensis and E. rutaecarpa water extract could significantly inhibit the formation of ACF in model animals. The proliferative crypts were increased obviously in middle and distal colon, and decreased by C. chinensis and E. rutaecarpa water extract. The apoptosis crypts were increased in distal colon but not middle colon. C. chinensis and E. rutaecarpa water extract could promote apoptosis of both middle and distal colon. Conclusion： C. chinensis and E. rutaecarpa water extract could significantly inhibit the formation of ACF in model animals. These results indicated that C. chinensis and E. rutaecarpa water extract maybe have an inhibitory and clinically therapeutic effect on colon cancer, which were partly resulted from inhibiting proliferation and promoting apoptosis of crypts in middle and distal colon.

关 键 词：黄连 吴茱萸 结肠癌 二甲肼 癌前病变 大鼠 

分 类 号：R285.5[医药卫生—中药学]